000 04543nam a22003855i 4500
999 _c393299
_d393299
001 393299
003 ES-MaUEC
005 20231216203255.0
006 a|||| o|||| 00| 0
007 cr nn 008mamaa
008 110414s2009 xxu| s |||| 0|eng d
020 _a9781603270175
024 7 _a10.1007/978-1-60327-017-5
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQH671
_b2009 EB
245 0 0 _aApoptosis :
_bMethods and Protocols, Second Edition
_cedited by P. Erhard, Ambrus Toth
250 _a2nd edition 2009
264 1 _aTotowa, NJ
_bHumana Press
_c2009
300 _a1 recurso en línea (XII, 400 páginas)
_b55 ilustraciones, 8 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v559
505 0 _aDetection of Apoptosis -- Analysing Caspase Activation and Caspase Activity in Apoptotic Cells -- Flow Cytometry-Based Apoptosis Detection -- Live to Dead Cell Imaging -- Detection of Apoptosis in Tissue Sections -- Detection of Apoptosis in Cell-Free Systems -- Detection of Non-Apoptotic Cell Death -- Methods to Analyze Cellular Necroptosis -- Detection of Cell Death by Autophagy -- Modifications of Apoptotic Proteins during Apoptosis -- Methods to Analyze Transglutamination of Proteins Involved in Apoptosis -- Methods to Analyze S-nitrosylation of Proteins Involved in Apoptosis -- Application of In Vivo EPR for Tissue pO2 and Redox Measurements -- Analysis of the Function of Major Regulators of Apoptosis/Cell Survival -- Assays to Measure p53-Dependent and -Independent Apoptosis -- Measurement of Changes in Cdk2 and Cyclin O-Associated Kinase Activity in Apoptosis -- Fluorometric Methods for Detection of Mitochondrial Membrane Permeabilization in Apoptosis -- Regulation of Apoptosis by the Unfolded Protein Response -- Detection of Uncoupling Protein-2 (UCP2) As a Mitochondrial Modulator of Apoptosis -- Multiple Approach to Analyzing the Role of MicroRNAs in Apoptosis -- Assessment of Apoptotic Cell Phagocytosis by Macrophages -- Analysis of Apoptosis in Different Organs -- Detection of Apoptosis in Mammalian Development -- Detection of Apoptosis in the Central Nervous System -- Genetic Mapping of Anti-Apoptosis Pathways in Myeloid Progenitor Cells -- Analysis of Apoptosis in Isolated Primary Cardiac Myocytes -- Cell Death in Myoblasts and Muscles -- Analysis of Apoptosis in Model Organisms -- Reliable Method for Detection of Programmed Cell Death in Yeast -- Detection of Cell Death in Drosophila -- Detecting Apoptotic Cells and Monitoring Their Clearance in the Nematode Caenorhabditis elegans -- Detection of Herpes Simplex Virus Dependent Apoptosis.
520 _aThe ability to detect and quantify apoptosis, to understand its biochemistry, and to identify its regulatory genes and proteins is crucial to biomedical research. In Apoptosis: Methods and Protocols, Second Edition, expert researchers describe the techniques to best investigate the critical steps involved in the apoptotic process. Presented from several different research perspectives, the volume contains sections covering detection of apoptosis, detection of non-apoptotic cell death, modifications of apoptotic proteins during apoptosis, the analysis of its major regulators, as well as analysis of apoptosis in different organs and in model organisms. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Apoptosis: Methods and Protocols, Second Edition constitutes a key technical reference to the significant methodologies used in the field, and offers beginners and experienced researchers powerful tools to illuminate the phenomena of programmed cell death.
988 _aSpringer_Protocols_2009
650 7 _2embne
_9157983
_aApoptosis
650 7 _2embne
_9160237
_aMuerte celular
650 7 _2embne
_9139103
_aBiología molecular
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60327-017-5
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b12/2023
_dz
_ejc
_zSI