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| 008 | 100301s1998 xxu| s |||| 0|eng d | ||
| 020 | _a9781592592609 | ||
| 024 | 7 |
_a10.1385/0896035212 _2doi |
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| 040 |
_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC |
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| 050 | 4 |
_aQR201 .H46 _b1998 EB |
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| 245 | 0 | 0 |
_aHepatitis C Protocols _cedited by Johnson Y. N. Lau |
| 250 | _a1st edition 1998 | ||
| 264 | 1 |
_aTotowa, NJ _bHumana Press _c1998 |
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| 300 |
_a1 recurso en línea (XXI, 621 páginas) _b171 ilustraciones |
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| 336 |
_atexto _btxt _2rdacontent |
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| 337 |
_aelectrónico _bc _2rdamedia |
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| 338 |
_arecurso electrónico _bcr _2rdacarrier |
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| 347 |
_aarchivo de texto _bPDF |
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| 490 | 0 |
_aMethods in Molecular Medicine _x1940-6037 _v19 |
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| 505 | 0 | _aDetection of Anti-HCV in Serum -- Detection of Anti-GOR Antibodies in HCV Infection -- Confirmation of HCV Antibodies by the Line Immunoassay INNO-LIA HCV Ab III -- Detection and Quantitation of HCV in Serum -- Detection of HCV RNA in Serum by Reverse Transcriptase-PCR and Radiolabeled Liquid Hybridization -- Detection of HCV RNA in Serum by Reverse Transcription Polymerase Chain Reaction (RT-PCR) -- The AMPLICOR® HCV Tests for the Detection and Quantitation of Serum or Plasma HCV RNA -- Quantification of HCV RNA in Clinical Specimens by Branched DNA (bDNA) Technology -- Quality Control of the Polymerase Chain Reaction -- Preparation of Genotype-Specific HCV RNA Transcripts for Assessing HCV Detection and Quantification Assays -- Detection of HCV in Liver Tissue -- Detection of HCV RNA in Formalin-Fixed, Paraffin-Embedded Liver Tissue by RT-PCR -- Quantification of HCV RNA in Liver Tissue by bDNA Assay -- HCV Genotypes -- Hepatitis C Virus -- Molecular Evolutionary Analysis -- Genotyping by Type-Specific Primers That Can Type HCV Types 1-6 -- Genotyping Hepatitis C Virus by Type-Specific Primers for PCR Based on NS5 Region -- Determination of HCV Genotypes by RFLP -- HCV Genotyping by the Line Probe Assay INNO-LiPA HCV II -- Serological Genotyping Using Synthetic Peptides Derived from the NS4 Region -- Determination of HCV Quasispecies by Cloning and Sequencing -- Detection of Hepatitis C Virus Quasispecies Heterogeneity by Single-Strand Conformational Polymorphism -- Hepatitis C Virus Heteroduplex Tracking Assay -- Detection of HCV In Situ -- In Situ Detection of HCV -- In Situ Detection of Hepatitis C Viral Antigens -- In Situ Hybridization and the Detection and Localization of HCV RNA -- The In Situ Detection of PCR-Amplified Hepatitis C RNA -- lmmunoelectron Microscopic Characterization of HCV -- Molecular Biologic Characterization -- Cloning and Assembly of Complex Libraries of Full-Length HCV cDNA Clones -- Use of the Vaccinia Virus/T7 Expression System for Studying HCV Protein Processing -- Use of a Discistronic Vector for the Quantitation of HCV IRES Activity -- Expression and Dimerization of Hepatitis C Virus Core Protein in E. coli -- Expression and Characterization of the HCV NS2 Protease -- Expression and Characterization of HCV NS3 Protease -- Expression and Characterization of the HCV NS3 Helicase Domain -- Hepatitis C Virus RNA-Dependent RNA Polymerase (NS5B Polymerase) -- Detection and Molecular Cloning of the Extreme 3?-End of HCV -- In Vitro Stability of Hepatitis C Virus RNA -- Methods for the Study of Sequence-Specific Binding of Proteins to the HCV RNA Genome -- HCV-Specific Immunologic Response -- Role of Immune Response in HCV -- Determination of Hepatitis C Virus-Specific CD4+ T-Cell Activity in PBMC -- Measurement of HCV-Specific CD8+ Cytotoxic T-Cell Activities in the Peripheral Blood by Europium Release Assay -- Determination of HCV-Specific Bulk CD8+ Activity in Liver -- Characterization of HCV-Specific Cytotoxic T-Lymphocytes from Liver Tissue -- Production of Human Monoclonal Antibodies to Hepatitis C Virus and Their Characterization -- In Vitro Culture Model -- Specific Detection of Negative Strand RNA of Hepatitis C Virus Using Chemical RNA Modification -- Strand-Specific rTth RT-PCR for the Analysis of HCV Replication -- Cell Culture Systems for the Detection of HCV Infection -- Replication and Detection of Hepatitis C Virus in Liver-Derived Cell Lines -- Primary Human Hepatocyte Culture for the Study of HCV -- A Cultivation Method for Highly Differentiated Primary Chimpanzee Hepatocytes Permissive for Hepatitis C Virus Replication -- Construction of Recombinant Viral Vectors for the Expression of HCV Genes -- Construction of Recombinant Vaccinia Virus Expressing HCV Genes -- Construction of Recombinant Adeno-Associated Virus (AAV) -- Production of Replication-Deficient Adenovirus Recombinants -- Generation of Recombinant Herpes Simplex Virus Ampiicons -- Construction of Recombinant Sindbis-Based Expression Vectors for the Study of HCV Genes and Their Products -- In Vivo Models -- The Chimpanzee Model -- Liver Transplantation as a Model to Study Hepatitis C Virus Infection. | |
| 520 | _aIn Hepatitis C Protocols, Johnson YN Lau and his expert collaborators present cutting-edge techniques for study of the hepatitis C virus infection and its disease, hepatitis C, which afflict an estimated 170 million people worldwide. The methods range from the detection of anti-HCV in serum and HCV in liver tissue, to HCV genotyping and the detection and quantification of HCV in serum. In addition to these key protocols, the authoritative contributors also evaluate recombinant proteins in immunological test systems, including the identification of important antigenic epitopes, antibodies, and recombinant antigens. Molecular and immunological techniques to study this virus and its infection, as well as animal models and recombinant virus technology commonly used to express hepatitis C viral proteins, are also developed. Hepatitis C Protocols summarizes the state-of-the-art in today's research and understanding of the virology of hepatitis C virus. The methods and systems detailed in this book will play a key role in the development of more effective antivirals, in the development of vaccines, and in a deeper understanding of viral replication strategies, viron structure, and the mechanisms of viron assembly and release. A valuable new standard resource for all researchers and clinicians, Hepatitis C Protocols leads the way toward improved prevention, diagnosis, and treatment of viral hepatitis C. | ||
| 988 | _aSpringer_Protocols_1998 | ||
| 650 | 7 |
_2embne _9158778 _aHepatitis C |
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| 650 | 7 |
_2embne _9138404 _aEnfermedades infecciosas |
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| 650 | 7 |
_2embne _9145703 _aHepatitis víricas |
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| 856 | 4 | 0 |
_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896035212 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid) |
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_2lcc _cLE |
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| 998 |
_b12/2023 _dz _ejc _zSI |
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