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008			140926s2015 xxu| s |||| 0|eng d
020			_a9781493915712
024	7		_a10.1007/978-1-4939-1571-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR410.2 .R45 _b2015 EB
245	1	0	_aRhinoviruses : _bMethods and Protocols _cedited by David A. Jans, Reena Ghildyal
250			_a1st edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (XII, 190 páginas) _b37 ilustraciones, 17 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1221
505	0		_aClassification and Evolution of Human Rhinoviruses -- Nested-RT-PCR and Multiplex RT-PCR for Diagnosis of Rhinovirus Infection in Clinical Samples -- Molecular Identification and Quantification of Human Rhinoviruses in Respiratory Samples -- Molecular Genotyping of Human Rhinovirus by PCR and Sanger Sequencing -- Growth of Human Rhinovirus in H1-Hela Cell Suspension Culture and Purification of Virions -- Propagation of Rhinovirus - C Strains in Differentiated Epithelial Cells of Human Airway at Air-Liquid Interface -- Infectivity Assays of Human Rhinovirus-A and -B Serotypes -- Application of FCS in Studies of Rhinovirus Receptor Binding and Uncoating -- Capillary Electrophoresis, Gas Phase Electrophoretic Mobility Molecular Analysis, and Electron Microscopy - Effective Tools for Quality Assessment and Basic Rhinovirus Research -- Proteases of Human Rhinovirus - Role in Infection -- A Protocol to Express and Isolate HRV16 3C Protease for Use in In vitro Protease Assays -- Reverse Genetics System for Studying Human Rhinovirus Infections -- Reverse Genetic Engineering of the Human Rhinovirus Serotype 16 Genome to Introduce an Antibody-Detectable Tag -- Mouse Models of Rhinovirus Infection and Airways Disease.
520			_aRhinoviruses: Methods and Protocols highlights the numerous molecular, cellular and in vivo tools now available to conduct human rhinovirus (HRV) research in an effort to increase understanding of the clinical disease caused by HRVs as well as the functions of its individual proteins and its replication. Human rhinoviruses are the major cause of common colds as well as being more recently recognized as the major viral cause of asthma and chronic obstructive pulmonary disease (COPD) exacerbations. The huge advancements in technical applications of biophysics and improvements in high-end imaging techniques have also had implications for HRV research among other infectious diseases. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Rhinoviruses: Methods and Protocols seeks to serve both professionals and novices with the latest technical advances in HRV research to ultimately enable the development of therapeutic approaches to combat HRV, and most importantly, its pathogenic effects in asthma exacerbation.
988			_aSpringer_Protocols_2015
650		7	_2embne _9413113 _aVirología
650		7	_2embne _9138684 _aMicrobiología
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-1571-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _ejc _zSI