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008			130716s2013 xxu| s |||| 0|eng d
020			_a9781627035620
024	7		_a10.1007/978-1-62703-562-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP609 .P5 _b2013 EB
245	0	0	_aPhosphatase Modulators _cedited by José Luis Millán
250			_a1st edition 2013
264		1	_aTotowa, NJ _bHumana Press _c2013
300			_a1 recurso en línea (X, 315 páginas) _b75 ilustraciones, 45 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1053
505	0		_aChemical Probe Development Versus Drug Development -- Phosphatase High-Throughput Screening Assay Design and Selection -- Multisystemic Functions of Alkaline Phosphatases -- Robotic Implementation of Assays: Tissue-Nonspecific Alkaline Phosphatase (TNAP) Case Study -- Inhibitors of Tissue-Nonspecific Alkaline Phosphatase (TNAP): From Hits to Leads -- A Method for Direct Assessment of Tissue-Nonspecific Alkaline Phosphatase (TNAP) Inhibitors in Blood Samples -- Isolation and Characteristics of Matrix Vesicles -- The Use of Tissue-Nonspecific Alkaline Phosphatase (TNAP) and PHOSPHO1 Inhibitors to Affect Mineralization by Cultured Cells -- Modulators of Intestinal Alkaline Phosphatase -- New Activity Assays for ENPP1 with Physiological Substrates ATP and ADP -- Structure of Acid Phosphatases -- Purification of Prostatic Acid Phosphatase (PAP) for Structural and Functional Studies -- Protein Tyrosine Phosphatases: Structure, Function, and Implication in Human Disease -- High-Throughput Screening for Protein Tyrosine Phosphatase Activity Modulators -- Evaluating Effects of Tyrosine Phosphatase Inhibitors on T Cell Receptor Signaling -- Interactor-Guided Dephosphorylation by Protein Phosphatase-1 -- Structure, Regulation, and Pharmacological Modulation of PP2A Phosphatases.
520			_aDriving further the research on mammalian alkaline phosphatase structure and function, Phosphatase Modulators collects expert contributions into one "how to" manual for basic scientists interested in initiating a drug discovery effort. While this book contains the traditional method chapters and some typical reviews on the structure and known functions of phosphatases, other contributions are meant to discuss approaches and alternatives useful in making "go/no-go" decisions in high throughput screening (HTS) and lead optimization campaigns. Many chapters focus on tissue-nonspecific alkaline phosphatase (TNAP) as well as protein phosphatases.  Written for the highly successful Methods in Molecular Biology series, chapters in this volume include the kind of detail and key implementation advice that promotes reproducible results.   Step-by-step and practical, Phosphatase Modulators offers a path to understanding many of the facets and complexities associated with undertaking a drug discovery effort and will serve as a roadmap to initiating those efforts.
988			_aSpringer_Protocols_2013
650		7	_2embne-DP _9676288 _aFosfatasa alcalina
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-562-0 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _ejc _zSI