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008			130321s2013 xxu| s |||| 0|eng d
020			_a9781627033459
024	7		_a10.1007/978-1-62703-345-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP356 _b2013 EB
245	0	0	_aChemical Neurobiology : _bMethods and Protocols _cedited by Matthew R. Banghart
250			_a1st edition 2013
264		1	_aTotowa, NJ _bHumana Press _c2013
300			_a1 recurso en línea (X, 202 páginas) _b70 ilustraciones, 1 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v995
505	0		_aEngineering K+ Channels using Semisynthesis -- Chemical Derivatization and Purification of Peptide-Toxins for Probing Ion Channel Complexes -- Using Yeast to Study Potassium Channel Function and Interactions with Small Molecules -- A FLIPR Assay for Evaluating Agonists and Antagonists of GPCR Heterodimers -- Characterizing Caged Molecules Through Flash Photolysis and Transient Absorption Spectroscopy -- Characterization of One- and Two- Photon Photochemical Uncaging Efficiency -- Photochromic Potassium Channel Blockers: Design and Electrophysiological Characterization -- A 1H NMR Assay for Measuring the Photostationary States of Photoswtichable Ligands -- Developing a Photoreactive Antagonist -- Development and in vitro Characterization of Ratiometric and Intensity-based Fluorescent Ion Sensors.-  Characterization of Voltage-Sensitive Dyes in Living Cells using Two-Photon Excitation -- Characterization and Validation of Fluorescent Receptor Ligands: A Case Study of the Ionotropic Serotonin Receptor.-  Imaging Single Synaptic Vesicles in Mammalian Central Synapses with Quantum Dots.-  Directed Evolution of Protein-Based Neurotransmitter Sensors for MRI.
520			_aMany advances in modern neuroscience are enabled by the availability of chemical tools that allow sensitive, precise, and quantitative measurements of, and control over, biological processes. These powerful reagents are widely used for investigating the nervous system at levels of detail ranging from ion channel structure to neural network dynamics. Recent advances in photochemistry, microscopy, and protein engineering have triggered a surge in the development and application of these interdisciplinary techniques. Chemical Neurobiology: Methods and Protocols assists with the design, characterization and validation of new chemical tools for neurobiology by providing detailed protocols of procedures and assays deemed essential for the successful development and implementation of such tools. Divided into three sections, topics cover chemical probes of membrane protein structure and function, photochemical control of protein and cellular function, and chemical probes for imaging in the nervous system. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Chemical Neurobiology: Methods and Protocols serves scientists at many levels, including students aiming to expand their perspective, laboratory researchers seeking technical guidance, and established investigators looking for creative solutions to their research problems in molecular, cellular and systems neuroscience. .
988			_aSpringer_Protocols_2013
650		7	_2embne _9139039 _aNeurobiología _vManuales de laboratorio
650		7	_2embne _9146728 _aNeuroquímica _vManuales de laboratorio
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-345-9 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _ejc _zSI