000			04099nam a22003735i 4500
999			_c393279 _d393279
001			393279
003			ES-MaUEC
005			20231211191547.0
006			a|||| o|||| 00| 0
007			cr nn 008mamaa
008			111205s2012 xxu| s |||| 0|eng d
020			_a9781617794278
024	7		_a10.1007/978-1-61779-427-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH450 _b2012 EB
245	0	0	_aNext-Generation MicroRNA Expression Profiling Technology : _bMethods and Protocols _cedited by Jian-Bing Fan
250			_a1st edition 2012
264		1	_aTotowa, NJ _bHumana Press _c2012
300			_a1 recurso en línea (XI, 341 páginas) _b64 ilustraciones, 11 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v822
505	0		_aControlling miRNA Regulation in Disease -- Introduction to miRNA Profiling Technologies and Cross-Platform Comparison -- Stem-Loop RT-qPCR for MicroRNA Expression Profiling -- Poly(T) Adaptor RT-PCR -- MicroRNA In Situ Hybridization -- Agilent MicroRNA Microarray Profiling System -- miRNA Gene Expression Profiling Using Illumina Universal BeadChips -- MicroRNA Expression Analysis Using the Affymetrix Platform -- Individualized miRNA Assay Panels Using Optically Encoded Beads -- Microfluidic Primer Extension Assay (MPEA) -- MicroRNA Profiling Using µParaflo Microfluidic Array Technology -- MicroRNA Expression Analysis Using the Illumina MicroRNA-Seq Platform -- Next Generation Sequencing of miRNAs with Roche 454 GS-FLX Technology: Steps for a Successful Application -- Methods for Small RNA Preparation for Digital Gene Expression Profiling by Next-Generation Sequencing -- Profiling of Short RNAs Using Helicos Single-Molecule Sequencing -- deepBase: Annotation and Discovery of microRNAs and Other Non-Coding RNAs from Deep Sequencing Data -- PhenomiR: MicroRNAs in Human Diseases and Biological Processes -- miRNA Expression Profiling: From Reference Genes to Global Mean Normalization -- miRNA Data Analysis: Next-Gen Sequencing -- Integrated miRNA Expression Analysis and Target Prediction -- miRNAs in Human Cancer -- Blood-Based miRNA Preparation for Non-Invasive Biomarker Development.
520			_aThe rapid pace of microRNA (miRNA) research continues to drive the advances of techniques for miRNA expression profiling, and innovative technologies that are more sensitive, specific, quantitative, and that are compatible with a wide range of biospecimens have been developed during the past few years.  In Next-Generation MicroRNA Expression Profiling Technology: Methods and Protocols, expert researchers in the field contribute detailed examinations of the most current approaches being used today.  This volume includes comprehensive coverage of methodologies that have been developed for miRNA profiling, as well as next-gen sequencing technology, miRNA databases, and specialized applications, such as cancer studies and miRNA-based non-invasive biomarker development.  Written in the highly successful Methods in Molecular Biology™ series format, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge, Next-Generation MicroRNA Expression Profiling Technology: Methods and Protocols provides an ideal guide for novices and experts alike who are seeking to utilize these powerful technologies.
988			_aSpringer_Protocols_2012
650		7	_2embne _9162770 _aExpresión génica
650		7	_2embne _9144940 _aARN
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-427-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _ejc _zSI