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008			100301s2004 xxu| s |||| 0|eng d
020			_a9781592597994
024	7		_a10.1385/1592597998 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQK882 _b2004 EB
245	0	0	_aPhotosynthesis Research Protocols _cedited by Robert Carpentier
250			_a1st edition 2004
264		1	_aTotowa, NJ _bHumana Press _c2004
300			_a1 recurso en línea (XIV, 346 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v274
505	0		_aFractionation of Thylakoid Membranes Into Grana and Stroma Thylakoids -- Isolation of Photosystem I Particles From Spinach -- Rapid Isolation and Purification of Photosystem I Chlorophyll-Binding Protein From Chlamydomonas reinhardtii -- Isolation of Photosystem II-Enriched Membranes and the Oxygen-Evolving Complex Subunit Proteins From Higher Plants -- Isolation of Functional Photosystem II Core Particles From the Cyanobacterium Synechocystis sp. PCC 6803 -- Isolation of Photosystem I Reaction Center Preparation From Spinach -- Isolation of Photosystem II Reaction Center Complexes From Plants -- Isolation of Photosystem I Reaction Center Subunit Polypeptides From Spinach -- Purification and Crystallization of the Cytochrome b 6 f Complex in Oxygenic Photosynthesis -- Purification of Plastocyanin and Cytochrome c 6 From Plants, Green Algae, and Cyanobacteria -- Preparation of Native and Recombinant Light-Harvesting Chlorophyll-a/b Complex -- Isolation and Characterization of Lamellar Aggregates of LHCII and LHCII-Lipid Macro-Assemblies With Light-Inducible Structural Transitions -- Separation, Purification, and Characterization of Polypeptide Composition of Subcomplexes of the Main Light-Harvesting Chlorophyll a/b-Protein Complex of Photosystem II -- Isolation of CP43 and CP47 Photosystem II Proximal Antenna Complexes From Plants -- The Determination and Quantification of Photosynthetic Pigments by Reverse Phase High-Performance Liquid Chromatography, Thin-Layer Chromatography, and Spectrophotometry -- Isolation and Identification of Chloroplast Lipids -- DNA Adducts With Chlorophyll and Chlorophyllin As Antimutagenic Agents -- Incorporation and Analysis of LHCII in Model Systems -- Photosystem II Reconstitution Into Proteoliposomes -- Extraction of the Functional Manganese and Calcium From Photosystem II -- Assay of Photoinhibition of Photosystem II and Protease Activity -- Thermoluminescence -- Detection of Free Radicals and Reactive Oxygen Species -- Stabilization of Photosynthetic Materials -- Determination of Phosphoproteins in Higher Plant Thylakoids -- Identifying Photoprotection Mutants in Arabidopsis thaliana -- A Simple Method for Chloroplast Transformation in Chlamydomonas reinhardtii -- The Construction of Gene Knockouts in the Cyanobacterium Synechocystis sp. PCC 6803 -- Gene Inactivation in the Cyanobacterium Synechococcus sp. PCC 7002 and the Green Sulfur Bacterium Chlorobium tepidum Using In Vitro-Made DNA Constructs and Natural Transformation.
520			_aBecause photosynthesis is at the heart of plant bioenergetics, fundamental to plant productivity and biomass, and a major factor in global climate, it is widely studied across the many fields it embraces. In Photosynthesis Research Protocols, well-known researchers describe in step-by-step detail a broad range of basic and advanced biochemical techniques for isolating, treating, and analyzing the photosynthetic materials and processes. The methods presented are fundamental for research work in agriculture, biochemistry, biotechnology, botany, cell biology, environmental science, forestry, plant genetics, plant molecular biology, photobiology, photophysics, photoprotection, and plant physiology. All protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Photosynthesis Research Protocols offers both newcomers and experienced researchers an up-to-date collection of readily reproducible techniques essential for photosynthesis research today.
988			_aSpringer_Protocols_2004
650		7	_2embne _9139651 _aFotosíntesis
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592597998 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _ejc _zSI