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008			140611s2014 xxu| s |||| 0|eng d
020			_a9781493909315
024	7		_a10.1007/978-1-4939-0931-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP623.5 .S63 _b2014 EB
245	0	0	_aAnimal Endo-SiRNAs : _bMethods and Protocols _cedited by Andreas Werner
250			_a1st edition 2014
264		1	_aNew York, NY _bSpringer International Publishing _c2014
300			_a1 recurso en línea (X, 182 páginas) _b36 ilustraciones, 24 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1173
505	0		_aTargeted Small Non-coding RNA Directed Gene Activation in Human Cells -- Isolation of Chromatoid Dodies from Mouse Testis as a Rich Source of Short RNAs -- Generation of Endo-siRNAs in Xenopus laevis Oocytes -- Analysis of Endo-siRNAs in Drosophila -- Methods for Studying the Biological Consequences of Endo-siRNA Deficiency in Drosophila melanogaster -- Small RNA Library Cloning Procedure for Deep Sequencing of Specific Endogenous siRNA Classes in Caenorhabditis elegans -- Assays for Direct and Indirect Effects of C. elegans Endo-siRNAs -- Extraction and Non-radioactive Detection of Small RNA Molecules -- p19 Mediated Enrichment and Detection of siRNAs -- Detection of Small non-coding RNAs by in situ Hybridization using Probes of 2'-O-Methyl RNA + LNA -- Enhanced Detection of Small RNAs using a non-Radioactive Approach -- Computing siRNA and piRNA Overlap Signatures -- Isolation of Small Interfering RNAs using Viral Suppressors of RNA Interference -- Computational Analysis, Biochemical Purification and Detection of tRNA-derived Small RNA Fragments -- Differential DNA Methylation Patterns in Endo-siRNAs Mediated Silencing of LINE-1 Retrotransposons.
520			_aAnimal Endo-SiRNAs: Methods and Protocols presents a variety of approaches to investigate endo-siRNAs.  These include protocols applicable to study short RNAs expressed at a low level and model systems that are particularly suitable to investigate specific aspects of endo-siRNAs, their synthesis, their genomics or regulatory role. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Animal Endo-SiRNAs: Methods and Protocols contains practical tips that are absent in standard lab manuals.  .
988			_aSpringer_Protocols_2014
650		7	_2embne _9144940 _aARN
650		7	_2embne _9140525 _aÁcidos nucleicos
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-0931-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _ejc _zSI