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020 _a9781627032698
024 7 _a10.1007/978-1-62703-269-8
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aRC280 .L9
_b2013 EB
245 0 0 _aLymphoma :
_bMethods and Protocols
_cedited by Ralf Küppers
250 _a1st edition 2013
264 1 _aTotowa, NJ
_bHumana Press
_c2013
300 _a1 recurso en línea (XI, 315 páginas)
_b46 ilustraciones, 24 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v971
505 0 _aOrigin and Pathogenesis of B Cell Lymphomas -- Flow Cytometry for Non-Hodgkin and Classical Hodgkin Lymphoma -- Laser-Based Microdissection of Single Cells from Tissue Sections and PCR Analysis of Rearranged Immunoglobulin Genes from Isolated Normal and Malignant Human B Cells -- PCR-Based Analysis of Rearranged Immunoglobulin or T-Cell Receptor Genes by GeneScan Analysis or Heteroduplex Analysis for Clonality Assessment in Lymphoma Diagnostics -- Expression Cloning of Human B Cell Immunoglobulins -- Studying the Replication History of Human B Lymphocytes by Real-Time Quantitative (RQ)-PCR -- The Detection of Chromosomal Translocations Involving the Immunoglobulin Loci in B-Cell Malignancies -- Stereotyped B Cell Receptors in B Cell Leukemias and Lymphomas -- Flow Cytometric MRD Detection in Selected Mature B-Cell Malignancies -- MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCR -- Enrichment of Methylated DNA by Methyl-CpG Immunoprecipitation -- Gene Expression Profile Analysis of Lymphomas -- FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas -- Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens -- Studying MicroRNAs in Lymphoma -- Molecular Methods of Virus Detection in Lymphoma -- High Throughput RNA Sequencing in B-Cell Lymphomas.Expression Cloning of Human B Cell Immunoglobulins -- Studying the Replication History of Human B Lymphocytes by Real-Time Quantitative (RQ)-PCR -- The Detection of Chromosomal Translocations Involving the Immunoglobulin Loci in B-Cell Malignancies -- Stereotyped B Cell Receptors in B Cell Leukemias and Lymphomas -- Flow Cytometric MRD Detection in Selected Mature B-Cell Malignancies -- MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCR -- Enrichment of Methylated DNA by Methyl-CpG Immunoprecipitation -- Gene Expression Profile Analysis of Lymphomas -- FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas -- Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens -- Studying MicroRNAs in Lymphoma -- Molecular Methods of Virus Detection in Lymphoma -- High Throughput RNA Sequencing in B-Cell Lymphomas.
520 _aLymphomas are lymphoid malignancies derived from B or T lymphocytes, and their study has been and still is paradigmatic for many aspects of cancer research.  Lymphoma: Methods and Protocols presents and discusses key methods that are used in lymphoma research, partly specific for lymphoma research but often adaptable to the study of other cancers. By covering a broad variety of methods used in lymphoma research, this book will be of interest not only for hematologists, hematopathologists, and immunologists but also for scientists interested in other fields of cancer research as well as human genetics. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Versatile and cutting-edge, Lymphoma: Methods and Protocols serves researchers studying human physiology with the ultimate goal of understanding and controlling these often terrible diseases.
988 _aSpringer_Protocols_2013
650 7 _2embne
_9668066
_aLinfomas
650 7 _2embne
_9144344
_aLinfocitos T
650 7 _2embne
_9675040
_aLinfocitos B
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-269-8
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b12/2023
_dz
_ejc
_zSI