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008			100301s2004 xxu| s |||| 0|eng d
020			_a9781592596553
024	7		_a10.1385/159259655X _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP551 _b2004 EB
245	0	0	_aProtein Purification Protocols _cedited by Paul Cutler
250			_a2nd edition 2004
264		1	_aTotowa, NJ _bHumana Press _c2004
300			_a1 recurso en línea (XII, 484 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v244
505	0		_aGeneral Strategies -- Preparation of Extracts From Animal Tissues -- Protein Extraction From Plant Tissues -- Extraction of Recombinant Protein From Bacteria -- Protein Extraction From Fungi -- Subcellular Fractionation of Animal Tissues -- Subcellular Fractionation of Plant Tissues -- The Extraction of Enzymes From Plant Tissues Rich in Phenolic Compounds -- Avoidance of Proteolysis in Extracts -- Concentration of Extracts -- Making and Changing Buffers -- Purification and Concentration by Ultrafiltration -- Bulk Purification by Fractional precipitation -- Ion-Exchange Chromatography -- Hydrophobic Interaction Chromatography -- Affinity Chromatography -- Dye-Ligand Affinity Chromatography -- Lectin Affinity Chromatography -- Immunoaffinity Chromatography -- Immobilized Metal-Ion Affinity Chromatography -- Chromatography on Hydroxyapatite -- Thiophilic Affinity Chromatography and Related Methods -- Affinity Precipitation Methods -- Isoelectric Focusing -- Chromatofocusing -- Size-Exclusion Chromatography -- Fast Protein Liquid Chromatography -- Reversed-Phase Chromatography of Proteins -- Extraction of Membrane Proteins -- Removal of Detergent From Protein Fractions -- Purification of Membrane Proteins -- Lyophilization of Proteins -- Storage of Pure Proteins -- Electroelution of Proteins From Polyacrylamide Gels -- Electroblotting of Proteins From Polyacrylamide Gels -- Two-Dimensional Polyarcylamide Gel Electrophoresis for Proteome Analyses -- Microscale Solution Isoelectrofocusing -- Practical Column Chromatography -- Detection Methods -- Peptide Proteomics -- Multidimensional Liquid Chromatography of Proteins -- Mass Spectrometry -- Purification of Therapeutic Proteins -- Purification Process Scale-Up.
520			_aIn this new edition of the very successful Protein Purification Protocols (1996), Paul Cutler completely updates the existing protocols to reflect recent advances and adds an enormous new array of proteomic techniques for protein isolation and analysis. These cutting-edge techniques include not only two-dimensional gel electrophoresis for analysis and characterization, but also analytical chromatography for multidimensional separations of proteins and peptides, and mass spectrometry for isolating proteins. With the many recent advances in technology, simple spectrometric detection is no longer the only option for separating proteins, and the authors treat in full detail all the newer methods for these separations. Comprehensive and highly practical, Protein Purification Protocols, Second Edition, brings together all the key methodologies that both novice and experienced investigators need to carry out successful experimental work on proteins and their functions today.
988			_aSpringer_Protocols_2004
650		7	_2embne _9139861 _aProteínas
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/159259655X _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _ejc _zSI