000			05066nam a22003735i 4500
999			_c393263 _d393263
001			393263
003			ES-MaUEC
005			20231210193006.0
006			a|||| o|||| 00| 0
007			cr nn 008mamaa
008			110803s2011 xxu| s |||| 0|eng d
020			_a9781617791703
024	7		_a10.1007/978-1-61779-170-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRC347.5 _b2011 EB
245	0	0	_aIn Vitro Neurotoxicology : _bMethods and Protocols _cedited by Lucio G. Costa, Gennaro Giordano, Marina Guizzetti
250			_a1st edition 2011
264		1	_aTotowa, NJ _bHumana Press _c2011
300			_a1 recurso en línea (XII, 415 páginas) _b66 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v758
505	0		_aIn Vitro Neurotoxicology: An Introduction -- Primary Neurons in Culture and Neuronal Cell Lines for In Vitro Neurotoxicological Studies -- Determination of Metal Interactions with the Chaperone Hspa5 in Human Astrocytoma Cells and Rat Astrocyte Primary Cultures -- Microglia Cell Culture: A Primer for the Novice -- Neural Stem Cells for Developmental Neurotoxicity Studies -- Preparation, Maintenance, and Use of Serum-Free Aggregating Brain Cell Cultures -- Neurospheres as a Model for Developmental Neurotoxicity Testing -- Acute Hippocampal Slice Preparation and Hippocampal Slice Cultures -- Glia-Neuron Sandwich Co-Cultures: An In Vitro Approach to Evaluate Cell-to-Cell Communication in Neuroinflammation and Neurotoxicity -- In Vitro Models to Study the Blood Brain Barrier -- Measurements of Cell Death in Neuronal and Glial Cells -- Measurements of Neuronal Apoptosis -- Measurement of Isoprostanes as Markers of Oxidative Stress -- Assessment of Glutathione Homeostasis -- Assessing Neuronal Bioenergetic Status -- Fluorescent Assessment of Intracellular Calcium in Individual Cells -- Homeostatic Regulation of Glutamate Neurotransmission in Primary Neuronal Cultures -- Detection of Nitric Oxide Formation in Primary Neural Cells and Tissues -- JNK3-Mediated Apoptotic Cell Death in Primary Dopaminergic Neurons -- Measurement of Proteasomal Dysfunction in Cell Models of Dopaminergic Degeneration -- Cell Signaling and Neurotoxicity: Protein Kinase C In Vitro and In Vivo -- Cell Signaling and Neurotoxicity: 3H-Arachidonic Acid Release (Phospholipase A2) in Cerebellar Granule Neurons -- Quantitative Assessment of Neurite Outgrowth in PC12 Cells -- Measurements of Astrocyte Proliferation -- Quantification of Synaptic Structure Formation in Co-Cultures of Astrocytes and Hippocampal Neurons -- Volume Measurements in Cultured Primary Astrocytes -- Assessment of Cholesterol Homeostasis in Astrocytes and Neurons -- Dose-Response or Dose-Effect Curves in In Vitro Experiments and Their Use to Study Combined Effects of Neurotoxicants.
520			_aIn recent years, the need to develop acceptable alternatives to conventional animal testing for neurotoxicity and developmental neurotoxicity has been increasingly recognized, and much effort is being directed toward the development of alternative models, utilizing mostly mammalian cells in culture but also non-mammalian model systems.  In Vitro Neurotoxicology: Methods and Protocols presents a series of cellular, biochemical, and molecular methodological protocols in the area of in vitro neurotoxicology, with an emphasis on mammalian cell culture systems.  Opening with a section on methodologies for preparing several cellular systems of variable complexity, amenable for in vitro neurotoxicological studies, the thorough volume continues with coverage of methods to measure cellular death and major mechanisms, methods for assessing mechanisms of nervous system cell toxicity related to impairment of cell signaling, while a final section illustrates additional methods for assessing important nervous system processes such as cell proliferation, neuritogenesis, and synaptogenesis.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Comprehensive and cutting-edge, In Vitro Neurotoxicology: Methods and Protocols serves researchers with an interest in assessing or characterizing the potential neurotoxicity of environmental contaminants, drugs, or other chemicals.
988			_aSpringer_Protocols_2011
650		7	_2embne _9159274 _aNeurotoxicología
650		7	_2embne _9158907 _aNeurociencias
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-170-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _ejc _zSI