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008			100715s2010 xxu| s |||| 0|eng d
020			_a9781607614043
024	7		_a10.1007/978-1-60761-404-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH585 _b2010 EB
245	0	0	_aLive Cell Imaging : _bMethods and Protocols _cedited by Dmitri Papkovsky
250			_a1st edition 2010
264		1	_aTotowa, NJ _bHumana Press _c2010
300			_a1 recurso en línea (XI, 367 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v591
505	0		_aI: General Principles and Overview -- Instrumentation for Live-Cell Imaging and Main Formats -- Labels and Probes for Live Cell Imaging: Overview and Selection Guide -- Live Cell Imaging: An Industrial Perspective -- II: Imaging Techniques, Probes, and Applications -- Design of Fluorescent Fusion Protein Probes -- Synthetic Fluorescent Probes for Imaging of Peroxynitrite and Hypochlorous Acid in Living Cells -- Photo-Activatable Probes for the Analysis of Receptor Function in Living Cells -- The Application of Fluorescent Probes for the Analysis of Lipid Dynamics During Phagocytosis -- Imaging of Mitotic Cell Division and Apoptotic Intra-Nuclear Processes in Multicolor -- Manipulation of Neutrophil-Like HL-60 Cells for the Study of Directed Cell Migration -- A Method for Analyzing Protein-Protein Interactions in the Plasma Membrane of Live B Cells by Fluorescence Resonance Energy Transfer Imaging as Acquired by Total Internal Reflection Fluorescence Microscopy -- Sample Preparation for STED Microscopy -- Two-Photon Permeabilization and Calcium Measurements in Cellular Organelles -- Imaging and Analysis of Three-Dimensional Cell Culture Models -- Long-Term Imaging in Microfluidic Devices -- Monitoring of Cellular Responses to Hypoxia -- Imaging of Cellular Oxygen and Analysis of Metabolic Responses of Mammalian Cells -- Analysis of Mitochondrial pH and Ion Concentrations -- Live Cell Imaging Analysis of Receptor Function -- Subcellular Dynamic Imaging of Protein-Protein Interactions in Live Cells by Bioluminescence Resonance Energy Transfer -- Quantitative Analysis of Membrane Potentials -- Image Correlation Spectroscopy to Define Membrane Dynamics.
520			_aNow a routine tool in biomedical and life science research, live cell imaging has made major progress enabling this core biochemical, cell, and molecular biology technique to become even more powerful, versatile, and affordable. In Live Cell Imaging: Methods and Protocols, a panel of expert contributors provide a comprehensive compendium of experimental approaches to live cell imaging in the form of several overview chapters followed by representative examples and case studies covering different aspects of the most current methodology. By examining a range of state-of-the-art protocols extensively validated in complex biological studies, this volume highlights new experimental and instrumental opportunities and helps researchers to select appropriate imaging methods for their specific biological questions and measurement tasks. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Live Cell Imaging: Methods and Protocols promises to contribute greatly to the further development and dissemination of this fundamentally important technology which spans across many disciplines including molecular and cell biology, chemistry, physics, optics, engineering, cell physiology, and medicine.
988			_aSpringer_Protocols_2010
650		7	_2embne _9139475 _aCélulas
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60761-404-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _ejc _zSI