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008			100301s2007 xxu| o |||| 0|eng d
020			_a9781597451871
024	7		_a10.1385/1597451878 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aTP248.65 .P76 _b2007 EB
245	0	0	_aProtein Engineering Protocols _cedited by Kristian Müller, Katja Arndt
250			_a1st edition 2007
264		1	_aTotowa, NJ _bHumana Press _c2007
300			_a1 recurso en línea (328 páginas) _b65 ilustraciones, 1 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v352
505	0		_aDesign and Computational Strategies for Protein Engineering -- Combinatorial Protein Design Strategies Using Computational Methods -- Global Incorporation of Unnatural Amino Acids in Escherichia coli -- Considerations in the Design and Optimization of Coiled Coil Structures -- Calcium Indicators Based on Calmodulin-Fluorescent Protein Fusions -- Design and Synthesis of Artificial Zinc Finger Proteins -- Monobodies -- Engineering Site-Specific Endonucleases -- Evolutionary Strategies for Protein Engineering -- Protein Library Design and Screening -- Protein Design by Binary Patterning of Polar and Nonpolar Amino Acids -- Versatile DNA Fragmentation and Directed Evolution With Nucleotide Exchange and Excision Technology -- Degenerate Oligonucleotide Gene Shuffling -- M13 Bacteriophage Coat Proteins Engineered for Improved Phage Display -- Ribosome-Inactivation Display System -- Compartmentalized Self-Replication -- Synthesis of Degenerated Libraries of the Ras-Binding Domain of Raf and Rapid Selection of Fast-Folding and Stable Clones With the Dihydrofolate Reductase Protein Fragment Complementation Assay -- A General Method of Terminal Truncation, Evolution, and Re-Elongation to Generate Enzymes of Enhanced Stability.
520			_aProtein engineering is a fascinating mixture of molecular biology, protein structure analysis, computation, and biochemistry, with the goal of developing useful or valuable proteins. Divided into two sections, Protein Engineering Protocols reviews rational protein design strategies and directed evolutionary techniques, and their impact on protein engineering. The first section presents design and computational strategies for protein engineering focusing on designing a protein with desired properties, including examples covering a wide range of engineering techniques, such as protein-protein interactions, DNA binding, antibody mimics, and enzymatic activity. The second section on evolutionary techniques provides step-by-step instructions on library design and statistical assessment of library quality. New methods for DNA shuffling as well as different selection strategies are also presented. Following the successful Methods in Molecular Biology™ series format, this volume provides a comprehensive guide to the methods used at every stage of the engineering process. By combining a thorough theoretical foundation with detailed protocols, Protein Engineering Protocols will be invaluable to all research workers in the area, from graduate students to senior investigators.
988			_aSpringer_Protocols_2007
650		7	_2embne _9485040 _aIngeniería bioquímica _vManuales de laboratorio
650		7	_2embne _9139861 _aProteínas _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781617373381
776	0	8	_iPrinted edition: _z9781588290724
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1597451878 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _eu _zSI