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020 _a9781627036764
024 7 _a10.1007/978-1-62703-676-4
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQH634.2
_b2014 EB
245 0 0 _aExocytosis Methods
_cedited by Peter Thorn
250 _a1st edition 2014
264 1 _aTotowa, NJ
_bHumana Press
_c2014
300 _a1 recurso en línea (XIII, 248 páginas)
_b70 ilustraciones, 36 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aNeuromethods
_x1940-6045
_v83
505 0 _aSolution Single-Vesicle Fusion Assay by Single-Molecule Alternating Laser Excitation -- Imaging the Stages of Exocytosis in Epithelial Type II Pneumocytes -- Carbon-Fibre Amperometry in the Study of Exocytosis -- Imaging of Insulin Exocytosis from Pancreatic Beta Cells -- Functional, Quantitative, and Super-Resolution Imaging and Spectroscopic Approaches for Studying Exocytosis -- Electrophysiologic Measurements of Membrane Capacitance in Hormone-Secreting Cells -- Measuring Exocytosis in Endocrine Tissue Slices -- Intravital Microscopy and Its Application to Study Regulated Exocytosis in the Exocrine Glands of Live Rodents -- Rapid Analysis of Synaptic Vesicle Endocytosis in Synaptosomes -- Isolated Neurohypophysial Terminals: Model for Depolarization-Secretion Coupling -- The Sea Urchin Egg and Cortical Vesicles as Model Systems to Dissect the Fast, Ca2+-Triggered Steps of Regulated Exocytosis.
520 _aBringing together techniques and methods currently being applied to the study of exocytosis, Exocytosis Methods collects chapters from experts in the field, examining this fundamental process essential to functions ranging from protein secretion to hormone release and neurotransmission.  The book begins with a section covering a range of techniques being applied to the study of single-vesicle fusion events, which are key in order to gain insight into the final steps of vesicle fusion.  The volume continues with several model systems that are being employed to unravel the complexities of exocytosis.  Written for the Neuromethods series, chapters included in this work present the kind of detail and vital implementation advice that leads to successful results.   Practical and authoritative, Exocytosis Methods seeks to promote the advent of new methods in microscopy and the development of new preparations, which would doubtlessly lead to many new and exciting discoveries in this field.
988 _aSpringer_Protocols_2014
650 7 _2embne
_9139475
_aCélulas
_vManuales de laboratorio
776 0 8 _iPrinted edition:
_z9781627036771
776 0 8 _iPrinted edition:
_z9781627036757
776 0 8 _iPrinted edition:
_z9781493963294
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-676-4
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b12/2023
_dz
_eu
_zSI