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020 _a9781493972197
024 7 _a10.1007/978-1-4939-7219-7
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aRC268.42
_b2017 EB
245 1 0 _aErbB Receptor Signaling :
_bMethods and Protocols
_cedited by Zhixiang Wang
250 _a1st edition 2017
264 1 _aNew York, NY
_bSpringer International Publishing
_c2017
300 _a1 recurso en línea (X, 303 páginas)
_b54 ilustraciones, 41 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1652
505 0 _aErbB Receptors and Cancer -- New Insights from Drosophila into the Regulation of EGFR Signaling -- C. elegans Vulva Induction: An In Vivo Model to Study Epidermal Growth Factor Receptor Signaling and Trafficking -- Targeting HER2 in Advanced Breast Cancer -- Methods to Investigate EGFR Ubiquitination -- Dimerization Assessment of Epithelial Growth Factor Family of Receptor Tyrosine Kinases by Using Cross-Linking Reagent -- Application of Immunofluorescence Staining to Study ErbB Family of Receptor Tyrosine Kinases -- Activation of Endosome-Associated Inert EGF Receptor Following Internalization -- Two-Pulse Endosomal Stimulation of Receptor Tyrosine Kinases Induces Cell Proliferation -- Study of EGFR Signalling/Endocytosis by Site-Directed Mutagenesis -- Using Percoll Gradient Fractionation to Study the Endocytic Trafficking of the EGFR -- Analysis of Epidermal Growth Factor Receptor-Induced Cell Motility by Wound Healing Assay -- Cell Cycle Synchronization of HeLa Cells to Assay EGFR Pathway Activation -- Analysis of Constitutive EGFR Signaling Regulating IRF3 Transcriptional Activity in Cancer Cells -- Measurement of Epidermal Growth Factor Receptor-Derived Signals Within Plasma Membrane Clathrin Structures -- Studying Non-Proliferative Roles for Egfr Signaling in Tissue Morphogenesis Using Dorsal Closure of the Drosophila Embryo -- Analysis of Epithelial-Mesenchymal Transition Induced by Overexpression of Twist -- Assessment of Specificity of an Adenovirus Targeted to HER3/4 -- Isolation of Human Mesenchymal Stem Cells for Studying ErbB Receptor Signaling.
520 _aThis volume contains protocols specifically designed for studying cell signaling mediated by ErbB receptors, all applicable to the study of a broad range of ErbB receptor-mediated signaling from basic research to clinic applications, from cultured cells to various animal models and primary cancer cells from patients. After some overviews, the book continues by covering common protocols for studying various aspects of ErbB receptor-mediated cell signaling, newly-developed methods in biomedical research that are also widely-used in the study of ErbB receptor signaling, epidermal growth factor receptor (EGFR) signaling in Drosophila, as well as protocols for studying ErbB receptor signaling in various animal model systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Detailed and easy-to-follow, ErbB Receptor Signaling: Methods and Protocols serves as an ideal guide for researchers who are studying the cell signaling mediated by ErbB receptors and other receptor tyrosine kinases.
988 _aSpringer_Protocols_2017
650 7 _2embne
_9158538
_aOncogenes
_vManuales de laboratorio
776 0 8 _iPrinted edition:
_z9781493972180
776 0 8 _iPrinted edition:
_z9781493972203
776 0 8 _iPrinted edition:
_z9781493984206
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7219-7
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b12/2023
_dz
_eu
_zSI