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008			110414s2009 xxu| o |||| 0|eng d
020			_a9781603274142
024	7		_a10.1007/978-1-60327-414-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH599 _b2009 EB
245	1	0	_aChromatin Immunoprecipitation Assays : _bMethods and Protocols _cedited by Philippe Collas
250			_a1st edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (X, 268 páginas) _b47 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v567
505	0		_aThe State-of-the-Art of Chromatin Immunoprecipitation -- Characterization and Quality Control of Antibodies Used in ChIP Assays -- The Fast Chromatin Immunoprecipitation Method -- ?ChIP: Chromatin Immunoprecipitation for Small Cell Numbers -- Fish'n ChIPs: Chromatin Immunoprecipitation in the Zebrafish Embryo -- Epitope Tagging of Endogenous Proteins for Genome-Wide Chromatin Immunoprecipitation Analysis -- Flow Cytometric and Laser Scanning Microscopic Approaches in Epigenetics Research -- Serial Analysis of Binding Elements for Transcription Factors -- Modeling and Analysis of ChIP-Chip Experiments -- Use of SNP-Arrays for ChIP Assays: Computational Aspects -- DamID: A Methylation-Based Chromatin Profiling Approach -- Chromosome Conformation Capture (from 3C to 5C) and Its ChIP-Based Modification -- Determining Spatial Chromatin Organization of Large Genomic Regions Using 5C Technology -- Analysis of Nascent RNA Transcripts by Chromatin RNA Immunoprecipitation -- Methyl DNA Immunoprecipitation -- Immunoprecipitation of Methylated DNA -- Erratum to: Chromosome Conformation Capture (from 3C to 5C) and Its ChIP-Based Modification.
520			_aOver the past twenty years, the development of chromatin immunoprecipitation, or ChIP, assays has immensely enhanced the biological significance of the multifaceted DNA-binding proteins. In Chromatin Immunoprecipitation Assays: Methods and Protocols, researchers deeply involved in the development and improvement of the field provide cutting-edge protocols devoted to the most recent progress in ChIP and related subjects. The thorough chapters involve topics such as the characterization of ChIP antibodies, ChIP methods for small cell numbers, fast ChIP protocols, assays adapted to various species and cell types, as well as several strategies for the analysis of genome-wide datasets, while also extending a bit beyond ChIP assays to cover immunoprecipitation-based DNA methylation analyses, determination of spatial chromatin organization of large genomic regions, and RNA immunoprecipitation. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easy-to-use, Chromatin Immunoprecipitation Assays: Methods and Protocols is a timely guide for all those who wish to further the development of this powerful tool and continue delving into the incredible complexity of the cell's biology.
988			_aSpringer_Protocols_2009
650		7	_2embne _9144121 _aCromatina _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781607612100
776	0	8	_iPrinted edition: _z9781603274135
776	0	8	_iPrinted edition: _z9781617797507
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60327-414-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _eu _zSI