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008			100301s1994 xxu| o |||| 0|eng d
020			_a9781592595235
024	7		_a10.1385/0896032744 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP552 .P4 _b1994 EB
100	1		_aDunn, Ben M. _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut
245	1	0	_aPeptide Analysis Protocols _cby Ben M. Dunn, Michael W. Pennington
250			_a1st edition 1994
264		1	_aTotowa, NJ _bHumana Press _c1994
300			_a1 recurso en línea (XII, 335 páginas) _b15 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v36
505	0		_aGel-Filtration Chromotography -- Ion-Exchange Chromatography -- Reversed-Phase HPLC -- Reversed-Phase High-Performance Liquid Chromatography -- Applications of Strong Cation-Exchange (SCX)-HPLC in Synthetic Peptide Analysis -- Principles and Practice of Peptide Analysis with Capillary Zone Electrophoresis -- Fast Atom Bombardment Mass Spectrometric Characterization of Peptides -- Sequence Analysis of Peptide Resins from Boc/Benzyl Solid-Phase Synthesis -- NMR Spectroscopy of Peptides and Proteins -- Techniques for Conjugation of Synthetic Peptides to Carrier Molecules -- Epitope Prediction Methods -- Epitope mapping of a protein using the Geysen (PEPSCAN) Procedure -- Analysis of Proteinase Specificity by Studies of Peptide Substrates -- Synthesis of Recombinant Peptides -- De Novo Design of Proteins -- Chemical Synthesis of the Aspartic Proteinase from Human Immunodeficiency Virus (HIV) -- Multiple and Combinatorial Peptide Synthesis.
520			_aAs the technology base for the preparation of increasingly c- plex peptides has improved, the methods for their purification and ana- sis have also been improved and supplemented. Peptide science routinely utilizes tools and techniques that are common to organic chemistry, p- tein chemistry, biophysical chemistry, enzymology, pharmacology, and molecular biology. A fundamental understanding of each of these areas is essential for interpreting all of the data that a peptide scientist may see. The purpose of Peptide Analysis Protocols is to provide the novice with sufficient practical information necessary to begin developing useful analysis and separation skills. Understanding and developing these skills will ultimately yield a scientist with broadened knowledge and good problem-solving abilities. Although numerous books that address d- ferent specialties, such as HPLC, FAB-MS, CE, and NMR, have been written, until now no single volume has reviewed all of these techniques with a focus on "getting started" in separation and analysis of peptides. This volume will also provide those who already possess practical knowledge of the more advanced aspects of peptide science with detailed applications for each of these protocols. Because the chapters have been written by researchers active in each of the fields that they discuss, a great deal of information on and insight into solution of real problems that they have encountered is presented. Examplary results are clearly demonstrated and discussed. For more advanced investi- tions, supplementary experiments are often suggested.
988			_aSpringer_Protocols_1994
650		7	_2embne _9146243 _aPéptidos _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781489940049
776	0	8	_iPrinted edition: _z9781489940032
776	0	8	_iPrinted edition: _z9780896032743
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896032744 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _eu _zSI