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008			100806s2010 xxu| o |||| 0|eng d
020			_a9781607612445
024	7		_a10.1007/978-1-60761-244-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRM301.25 _b2010 EB
245	0	0	_aLigand-Macromolecular Interactions in Drug Discovery : _bMethods and Protocols _cedited by Ana Cecília A. Roque
250			_a1st edition 2010
264		1	_aTotowa, NJ _bHumana Press _c2010
300			_a1 recurso en línea (X, 298 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v572
505	0		_aReviews -- An Historical Overview of Drug Discovery -- Ligand Macromolecule Interactions: Theoretical Principles of Molecular Recognition -- X-Ray Crystallography in Drug Discovery -- Virtual Screening of Compound Libraries -- Protocols -- Combinatorial Chemistry and the Synthesis of Compound Libraries -- Ligand-Based Nuclear Magnetic Resonance Screening Techniques -- Isothermal Titration Calorimetry and Differential Scanning Calorimetry -- Adaptive Combinatorial Design of Focused Compound Libraries -- Chemical Microarrays: A New Tool for Discovery Enzyme Inhibitors -- Fluorescence Polarization and Time-Resolved Fluorescence Resonance Energy Transfer Techniques for PI3K Assays -- Small Molecule Protein Interaction Profiling with Functional Protein Microarrays -- Capillary Electrophoresis in Drug Discovery -- SPR in Drug Discovery: Searching Bioactive Compounds in Plant Extracts -- Application of Frontal Affinity Chromatography with Mass Spectrometry (FAC#x2013;MS) for Stereospecific Ligand#x2013;Macromolecule Interaction, Detection and Screening -- GPC Spin Column HPLC#x2013;ESI-MS Methods for Screening Drugs Noncovalently Bound to Proteins -- A Scintillation Proximity Assay for Fatty Acid Amide Hydrolase Compatible with Inhibitor Screening -- A Natural Products Approach to Drug Discovery: Probing Modes of Action of Antitumor Agents by Genome-Scale cDNA Library Screening -- Ligand#x2013;Macromolecule Interactions in Live Cells by Fluorescence Correlation Spectroscopy.
520			_aDrug research has been greatly transformed by the "omics revolution" and advances in computational tools, combinatorial chemistry, and high throughput screening techniques (HTS). These implements have advanced the ability to identify target molecules that serve as points of attack for future medicines, the design and synthesis of potential lead compounds, and further characterization, screening, and assays for therapeutic efficacy and toxicity. In Ligand-Macromolecular Interactions in Drug Discovery: Methods and Protocols, experts in the field highlight the main principles and methodologies currently utilized in the study of molecular interactions between compounds, either natural or synthetic, and complementary biological targets, within the scope of drug discovery. Beginning with a historical perspective of drug research focusing on the contribution of genomics, proteomics, high-throughput methods, and computational developments in drug discovery, the book then delves into highly detailed methods on topics such as NMR spectroscopy, compound library design and synthesis, chemical and biological microarrays, and many others. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Ligand-Macromolecular Interactions in Drug Discovery: Methods and Protocols will serve as an ideal guide to scientists in academia and in industry who are striving to further our knowledge of medicines.
988			_aSpringer_Protocols_2010
650		7	_2embne _9183058 _aFarmacología _xInvestigación
776	0	8	_iPrinted edition: _z9781607612452
776	0	8	_iPrinted edition: _z9781607612438
776	0	8	_iPrinted edition: _z9781493961771
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60761-244-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _eu _zSI