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008			100301s2001 xxu| o |||| 0|eng d
020			_a9781592590698
024	7		_a10.1385/1592590691 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_a QP551 _b2001 EB
245	0	0	_aKinesin Protocols _cedited by Isabelle Vernos
250			_a1st edition 2001
264		1	_aTotowa, NJ _bHumana Press _c2001
300			_a1 recurso en línea (XI, 258 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v164
505	0		_aPurification of Kinesin from the Brain -- RT-PCR for the Identification of Developmentally Regulated Novel Members of the Kinesin-like Superfamily -- Expression Cloning with Pan Kinesin Antibodies -- Expression of Kinesin in Escherichia coli -- Plasmids for Expression of Chimeric and Truncated Kinesin Proteins -- Preparation of Recombinant Kinesin Superfamily Proteins Using the Baculovirus System -- Assays for Kinesin Microtubule-Stimulated AT Pase Activity -- An Improved Microscope for Bead and Surface-Based Motility Assays -- Use of Photonic Force Microscopy to Study Single-Motor-Molecule Mechanics -- Assays for Microtubule-Destabilizing Kinesins -- Green Fluorescent Protein as a Tag for Molecular Motor Proteins -- In Vitro Reconstitution of Endosome Motility Along Microtubules -- Approaches to Study Interactions Between Kinesin Motors and Membranes -- Microinjection Methods for Analyzing the Functions of Kinesins in Early Embryos -- The Use of Dominant Negative Mutants to Study the Function of Mitotic Motors in the In Vitro Spindle Assembly Assay in Xenopus Egg Extracts -- A Dominant Negative Approach for Functional Studies of the Kinesin II Complex -- Identification of Kinesin-Associated Proteins -- Assaying Spatial Organization of Microtubules by Kinesin Motors -- Crystallization of Kinesin -- Structural Analysis of the Microtubule-Kinesin Complex by Cryo-Electron Microscopy.
520			_aIt is now clear that kinesin-like-proteins (KLP), generally thought to be responsible for the transport of cellular cargoes, are involved in many different cellular processes now being widely investigated. In Kinesin Protocols, Isabelle Vernos and a panel of hands-on experts present their most productive and reproducible techniques for the identification, purification, and characterization of the kinesin superfamily of microtubule-dependent motors. The methods range from the most basic to the most sophisticated and include step-by-step instructions and extensive cautionary notes to ensure experimental success. Among the approaches discussed are methods to express and purify kinesins in different systems, to characterize microtubule-enhanced ATPase activity and motility properties, and to test microtubule destabilizing activity. Detailed examples of how to address functional studies are also presented, along with some very new methods for studying the role of KLP in the organization of microtubules in three dimensions. There are also advanced methods for the study of kinesins at the structural level. Comprehensive and highly practical, Kinesin Protocols makes available all the key basic and cutting-edge methods needed successfully to study the multifaceted world of kinesin-like proteins and to explore their many functions.
988			_aSpringer_Protocols_2001
650		7	_2embne _9139861 _aProteínas _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781617371783
776	0	8	_iPrinted edition: _z9780896037663
776	0	8	_iPrinted edition: _z9781489941954
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592590691 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b11/2023 _da _eu _zSI