000			04919nam a2200397 i 4500
999			_c393201 _d393201 _x1
001			393201
003			ES-MaUEC
005			20240314174347.0
006			a|||| o|||| 00| 0
007			cr nn 008mamaa
008			100301s2000 xxu| o |||| 0|eng d
020			_a9781592590544
024	7		_a10.1385/1592590543 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP82.2 .S8 _b2000 EB
245	0	0	_aStress Response : _bMethods and Protocols _cedited by Stephen M. Keyse
250			_a1st edition 2000
264		1	_aTotowa, NJ _bHumana Press _c2000
300			_a1 recurso en línea (XVI, 488 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v99
505	0		_aDetection and Assay of Stress-Induced Damage -- Identifying and Counting Protein Modifications Triggered by Nitrosative Stress -- Determination of Carbonyl Groups in Oxidized Proteins -- Quantitation of 4-Hydroxynonenal Protein Adducts -- Detection of Oxidative Stress in Lymphocytes Using Dichlorodihydrofluorescein Diacetate -- The Measurement of Protein Degradation in Response to Oxidative Stress -- The Activation of Signal Transduction by Cellular Stress -- Analysis of the Role of the AMP-Activated Protein Kinase in the Response to Cellular Stress -- Detection and Activation of Stress-Responsive Tyrosine Kinases -- Detection of DNA-Dependent Protein Kinase in Extracts from Human and Rodent Cells -- Expression and Assay of Recombinant ATM -- Detection and Purification of a Multiprotein Kinase Complex from Mammalian Cells -- Methods to Assay Stress-Activated Protein Kinases -- Monitoring the Activation of Stress-Activated Protein Kinases Using GAL4 Fusion Transactivators -- Use of Kinase Inhibitors to Dissect Signaling Pathways -- The Development and Use of Phospho-Specific Antibodies to Study Protein Phosphorylation -- Peptide Assay of Protein Kinases and Use of Variant Peptides to Determine Recognition Motifs -- The Analysis of Stress-Induced Gene Expression -- Assaying NF-KB and AP-1 DNA-Binding and Transcriptional Activity -- Analysis of the Mammalian Heat-Shock Response -- Approaches to Define the Involvement of Reactive Oxygen Species and Iron in Ultraviolet-A Inducible Gene Expression -- The Human Immunodeficiency Virus LTR-Promoter Region as a Reporter of Stress-Induced Gene Expression -- Sage: The Serial Analysis of Gene Expression -- Analysis of Differential Gene Expression Using the SABRE Enrichment Protocol -- UVB-Regulated Gene Expression in Human Keratinocytes -- Analysis of Stress Protein Function -- Heme Oxygenase Activity Current Methods and Applications -- Analysis of Molecular Chaperone Activities Using In Vitro and In Vivo Approaches -- Analysis of Chaperone Properties of Small Hsp' -- Analysis of Small Hsp Phosphorylation -- Analysis of Multisite Phosphorylation of the p53 Tumor-Suppressor Protein byTryptic Phosphopeptide Mappin -- Development of Physiological Models to Study Stress Protein Responses.
520			_aIn is now understood that the response of mammalian cells to a wide variety of potentially toxic agents may be intimately linked with many human diseases, including rheumatoid arthritis, ischemia, fever, infection, and cancer. In Stress Response: Methods and Protocols, Stephen Keyse has assembled a diverse collection of readily reproducible methods devoted to the study of these varied and powerful responses. Written by leading researchers expert in the techniques they describe, these detailed methods cover the detection and assay of stress-induced damage, the activation of a wide range of signal transduction pathways by cellular stress, stress-induced gene expression, and stress protein function. To ensure experimental success, step-by-step guidance is provided for each method, along with details of reagents, equipment, and other requirements. The methods include both well-established techniques and new technologies at the leading edge of research. Wide ranging and highly practical, Stress Response: Methods and Protocols provides a gold-standard bench manual for today's basic and clinical scientists working to understand how cells and tissues respond during physiological stress and in human disease.
988			_aSpringer_Protocols_2000
650		7	_2embne _9146565 _aEstrés (Fisiología) _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781617370977
776	0	8	_iPrinted edition: _z9781489941916
776	0	8	_iPrinted edition: _z9780896036116
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592590543 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eb _zSI