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007			cr nn 008mamaa
008			100301s2008 xxua o |||| 0|eng d
020			_a9781597452342
024	7		_a10.1007/978-1-59745-234-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR376 _b2008 EB
245	0	0	_aPrion Protein Protocols _cedited by Andrew F. Hill
250			_a1st edition 2008
264		1	_aTotowa, NJ _bHumana Press _c2008
300			_a1 recurso en línea (XIV, 280 páginas) _b43 ilustraciones, 5 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v459
505	0		_aCell Culture Models to Unravel Prion Protein Function and Aberrancies in Prion Diseases -- Investigation of PrPC Metabolism and Function in Live Cells -- Immunodetection of PrPSc Using Western and Slot Blotting Techniques -- Assaying Prions in Cell Culture -- Generation of Cell Lines Propagating Infectious Prions and the Isolation and Characterization of Cell-derived Exosomes -- Neurotoxicity of Prion Peptides on Cultured Cerebellar Neurons -- Understanding the Nature of Prion Diseases Using Cell-free Assays -- Methods for Conversion of Prion Protein into Amyloid Fibrils -- Amplification of Purified Prions In Vitro -- Expression and Purification of Full-Length Recombinant PrP of High Purity -- Analysis of PrP Conformation Using Circular Dichroism -- Effect of Copper on the De Novo Generation of Prion Protein Expressed in Pichia pastoris -- Biophysical Investigations of the Prion Protein Using Electron Paramagnetic Resonance -- Molecular Diagnosis of Human Prion Disease -- Analysis of Endogenous PrPC Processing in Neuronal and Non-neuronal Cell Lines -- Molecular Typing of PrPres in Human Sporadic CJD Brain Tissue -- Transgenic Mouse Models of Prion Diseases -- Quantitative Bioassay of Surface-bound Prion Infectivity.
520			_aWhile much information has been gained over the last two decades regarding the molecular nature of prion proteins, only recently have several research groups begun to cast light on turning the normal prion protein into its aberrant, infectious form. In Prion Protein Protocols, an international team of experts provide an up-to-date collection of current methods in this unique area of neuroscience. The chapters contained in this volume, both protocols and useful review chapters, feature topics such as the cell biology of prions, techniques and approaches to studying prion infection in cultured cells, how these systems can be used as a rapid bioassay, and prion protein misfolding. As a volume in the highly successful Methods in Molecular Biology™ series, the chapters include readily reproducible, step-by-step laboratory protocols, lists of the necessary materials and reagents, and the Notes section, which provides tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Prion Protein Protocols is an ideal collection for researchers investigating the growing field of mammalian prion disease.
988			_aSpringer_Protocols_2008
650		7	_2embne _9413113 _aVirología _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781617378317
776	0	8	_iPrinted edition: _z9781588298973
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-234-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eb _zSI