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008			100427s2010 xxu| o |||| 0|eng d
020			_a9781607614616
024	7		_a10.1007/978-1-60761-461-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR185.8 .T2 _b2010 EB
245	0	0	_aT-Cell Trafficking : _bMethods and Protocols _cedited by Federica M. Marelli-Berg, Sussan Nourshargh
250			_a1st edition 2010
264		1	_aTotowa, NJ _bHumana Press _c2010
300			_a1 recurso en línea (XII, 290 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v616
505	0		_aIntroductory Review -- How T Cells Find Their Way Around -- Migration of T Cells In Vitro -- Live Imaging of Leukocyte-Endothelium Interactions -- Leucocyte Adhesion Under Haemodynamic Flow Conditions -- Influence of Stromal Cells on Lymphocyte Adhesion and Migration on Endothelial Cells -- Discriminating Between the Paracellular and Transcellular Routes of Diapedesis -- Monitoring RhoGTPase Activity in Lymphocytes -- Visualisation of Signalling in Immune Cells -- Methods for Quantitation of Leukocyte Chemotaxis and Fugetaxis -- Analysis of CXCR3 and Atypical Variant Expression and Signalling in Human T Lymphocytes -- Transfection of Indoleamine 2,3 Dioxygenase in Primary Endothelial Cells -- Migration of T Cells In Vivo -- Visualisation of Lymphoid Organ Development -- Single-Cell Analysis of Cytotoxic T Cell Function by Intravital Multiphoton Microscopy -- Imaging Interactions Between the Immune and Cardiovascular Systems In Vivo by Multiphoton Microscopy -- Applying an Adaptive Watershed to the Tissue Cell Quantification During T-Cell Migration and Embryonic Development -- Monitoring T-Cell Migration in Human Diseases -- Identifying Homing Interactions in T-Cell Traffic in Human Disease -- Tracking Antigen-Experienced Effector T Cells In Vitro and In Vivo -- Preclinical Testing of Strategies for Therapeutic Targeting of Human T-Cell Trafficking In Vivo.
520			_aIn the last decade, a large number of major discoveries have shed light on the molecular mechanisms of lymphocyte migration and the anatomy of immune responses. In T-Cell Trafficking: Methods and Protocols, expert researchers explore how the development of novel and cutting-edge techniques, particularly in the field of real-time imaging and genetic manipulation, have led to an increased understanding of lymphocyte trafficking. Written by internationally recognized experts in their respective fields, chapters provide state-of-the-art protocols to study lymphocyte migration and T-cell: endothelial cell interactions in vitro, address various approaches used for direct visualization of the development of the lymphoid system, lymphocyte recirculation, and effector responses in experimental models in vivo, and explore lymphocyte migration and inflammation in the human system. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Innovative and highly practical, T-Cell Trafficking: Methods and Protocols is an essential manual for newcomers in this ever-expanding and exciting area of research, as well as a valuable addition to more specialized laboratories.
988			_aSpringer_Protocols_2010
650		7	_2embne _9144344 _aLinfocitos T _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781607614647
776	0	8	_iPrinted edition: _z9781607614609
776	0	8	_iPrinted edition: _z9781493957040
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60761-461-6 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eb _zSI