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008			161114s2017 xxua o |||| 0|eng d
020			_a9781493964215
024	7		_a10.1007/978-1-4939-6421-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aTK8360 .O67 _b2017 EB
245	0	0	_aOptical Tweezers : _bMethods and Protocols _cedited by Arne Gennerich
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XII, 555 páginas) _b154 ilustraciones, 133 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1486
505	0		_a Introduction to Optical Tweezers -- Exact Theory of Optical Tweezers and its Application to Absolute Calibration -- Beyond the Hookean Spring Model: Direct Measurement of Optical Forces Through Light Momentum Changes -- A Surfaced-Coupled Optical Trap with 1-bp Precision via Active Stabilization -- Implementation and Tuning of an Optical Tweezers Force-Clamp Feedback System -- Custom-Made Microspheres for Optical Tweezers -- Optical Torque Wrench Design and Calibration -- High-Resolution 'Fleezers': Dual-Trap Optical Tweezers Combined with Single-Molecule Fluorescence Detection -- Versatile Quadruple-Trap Optical Tweezers for Dual DNA Experiments -- Probing DNA-DNA Interactions with a Combination of Quadruple-Trap Optical Tweezers and Microfluidics -- Probing Single Helicase Dynamics on Long Nucleic Acids Through Force-Fluorescence Measurement -- Mechanically Watching the ClpXP Proteasome Machinery -- Deciphering the Molecular Mechanism of the Bacteriophage Φ29 DNA Packaging Motor -- Single-Molecule Protein Folding Experiments using High-Precision Optical Tweezers -- Observing Single RNA Polymerase Molecules Down to Base-Pair Resolution -- Optical Tweezers-Based Measurements of Forces Generated by Dynamic Microtubule Ends -- Simultaneous Manipulation and Super-Resolution Fluorescence Imaging of Individual Kinetochores Coupled to Microtubule Tips -- Measurement of Force-Dependent Release Rates of Cytoskeletal Motors -- Measuring The Kinetic and Mechanical Properties of Non-Processive Myosins using Optical Tweezers -- Quantifying Force and Viscoelasticity Inside Living Cells Using an Active-Passive Calibrated Optical Trap -- Measuring Molecular Forces using Calibrated Optical Traps in Living Cells. .
520			_aThe aim of this volume is to provide a comprehensive overview of optical tweezers setups, both in practical and theoretical terms, to help biophysicists, biochemists, and cell biologists to build and calibrate their own instruments and to perform force measurements on mechanoenzymes both in isolation in vitro and in living cells. Chapters have been divided in three parts focusing on theory and practical design of optical tweezers, detailed protocols for performing force measurements on single DNA- and microtubule/actin-associated mechanoenzymes in isolation, and describing recent advances that have opened up quantitative force measurements in living cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Optical Tweezers: Methods and Protocols aims help to further expand the accessibility and use of optical traps by scientists of diverse disciplines. .
988			_aSpringer_Protocols_2017
650		7	_2embne _9146216 _aSensores ópticos _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781493964192
776	0	8	_iPrinted edition: _z9781493964208
776	0	8	_iPrinted edition: _z9781493981946
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6421-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eb _zSI