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020 _a9781592595839
024 7 _a10.1385/1592595839
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQK617.5
_b1999 EB
245 0 0 _aPlant Cell Culture Protocols
_cedited by Robert D. Hall
250 _a1st edition 1999
264 1 _aTotowa, NJ
_bHumana Press
_c1999
300 _a1 recurso en línea (XIV, 421 páginas)
_b33 ilustraciones
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v111
505 0 _aAn Introduction to Plant-Cell Culture -- Cell Culture and Plant Regeneration -- Callus Initiation, Maintenance, and Shoot Induction in Rice -- Callus Initiation, Maintenance, and Shoot Induction in Potato -- Somatic Embryogenesis in Barley Suspension Cultures -- Somatic Embryogenesis in Picea Suspension Cultures -- Direct, Cyclic Somatic Embryogenesis of Cassava for Mass Production Purposes -- Immature Inflorescence Culture of Cereals -- Cryopreservation of Rice Tissue Cultures -- Noncryogenic, Long-Term Germplasm Storage -- Plant Propagation In Vitro -- Micropropagation of Strawberry via Axillary Shoot Proliferation -- Meristem-Tip Culture for Propagation and Virus Elimination -- Clonal Propagation of Orchids -- In Vitro Propagation of Succulent Plants -- Micropropagation of Flower Bulbs -- Clonal Propagation of Woody Species -- Spore-Derived Axenic Cultures of Ferns as a Method of Propagation -- Applications for Plant Protoplasts -- Protoplast Isolation, Culture, and Plant Regeneration from Passiflora -- Isolation, Culture, and Plant Regeneration of Suspension-Derived Protoplasts of Lolium -- Protoplast Fusion for Symmetric Somatic Hybrid Production in Brassicaceae -- Production of Cybrids in Rapeseed (Brassica napus) -- Microprotoplast-Mediated Chromosome Transfer (MMCT) for the Direct Production of Monosomic Addition Lines -- Guard Cell Protoplasts -- In Vitro Fertilization with Isolated Single Gametes -- Protocols for Genomic Manipulation -- Protocols for Anther and Microspore Culture of Barley -- Microspore Embryogenesis and In Vitro Pollen Maturation in Tobacco -- Embryo Rescue Following Wide Crosses -- Mutagenesis and the Selection of Resistant Mutants -- The Generation of Plastid Mutants In Vitro -- Protocols for the Introduction of Specific Genes -- Agrobacterium-Mediated Transformation of Petunia Leaf Disks -- Transformation of Rice via PEG-Mediated DNA Uptake into Protoplasts -- Transformation of Wheat via Particle Bombardment -- Plant Transformation via Protoplast Electroporation -- Transformation of Maize via Tissue Electroporation -- Transformation of Maize Using Silicon Carbide Whiskers -- Suspension Culture Initiation and the Accumulation of Metabolites -- Directing Anthraquinone Accumulation via Manipulation of Morinda Suspension Cultures -- Alkaloid Accumulation in Catharanthus roseus Suspension Cultures -- Betalains.
520 _aIn Plant Cell Culture Protocols, Robert Hall and a panel of expert researchers present a comprehensive collection of the most frequently used and broadly applicable techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods cover culture initiation, maintenance, manipulation, application, and long-term storage, with emphasis on techniques for genetic modification and micropropagation. Many of these protocols are currently used in major projects designed to produce improved varieties of important crop plants. In addition, a number of specialized protocols have been included to illustrate the diversity of the techniques available and their widespread applicability. Plant Cell Culture Protocols is aimed at scientists involved in all aspects of plant biotechnological research, as well as those working in other areas of agriculture and horticulture who are interested in expanding their technical repertoire to include in vitro methodology. Its state-of-the-art techniques are certain to make the book today's reference of choice, an indispensable tool in the development of new transgenic plants and full-scale commercial applications.
988 _aSpringer_Protocols_1999
650 7 _2embne
_9163872
_aLevaduras (Botánica)
776 0 8 _iPrinted edition:
_z9781489943606
776 0 8 _iPrinted edition:
_z9780896035492
776 0 8 _iPrinted edition:
_z9781489943590
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592595839
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b01/2024
_dz
_eb
_zSI