000			04411nam a2200397 i 4500
999			_c393183 _d393183 _x1
001			393183
003			ES-MaUEC
005			20240314174341.0
006			a|||| o|||| 00| 0
007			cr nn 008mamaa
008			100301s2001 xxu| o |||| 0|eng d
020			_a9781592590841
024	7		_a10.1385/1592590845 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRC280 .C6 _b2001 EB
245	0	0	_aColorectal Cancer : _bMethods and Protocols _cedited by Steven M. Powell
250			_a1st edition 2001
264		1	_aTotowa, NJ _bHumana Press _c2001
300			_a1 recurso en línea (XIII, 294 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Medicine _x1940-6037 _v50
505	0		_aMicrodissection of Histologic Sections -- Isolation of a Purified Epithelial Cell Population from Human Colon -- Xenografting Human Colon Cancers -- Comparative Genomic Hybridization Technique -- Fluorescence In Situ Hybridization -- Microsatellite Analysis to Assess Chromosome 18q Status in Colorectal Cancer -- The SURF Technique -- Microsatellite Instability Testing -- Immunohistochemical Analysis for hMLH1 and hMSH2 Expression in Colorectal Cancer -- Mutation Detection in Colorectal Cancers -- Mutation Detection inTGF-? Receptors -- Direct Analysis for Familial Adenomatous Polyposis Mutations -- I1307K Mutation Detection by Allele-Specific PCR in Familial Colorectal Cancer -- Microsatellite Analysis of the Insulin-Like Growth Factor II Receptor in Colorectal Carcinomas -- Molecular Detection of Smad2/Smad4 Alterations in Colorectal Tumors -- Direct Sequencing for Juvenile Polyposis Gene SMAD4/DPC4 Mutations -- 17 Direct Sequencing for Peutz-Jeghers Gene LKB1 (STK11) Mutations -- 18 Direct Sequencing for Cowden Syndrome Gene PTEN (MMAC1) Mutations -- Genetic Analysis Using Microarrays -- Cclooxygenase-2 (COX-2) Protein Expression by Western Blotting -- The Immunohistochemical Method -- Immunohistochemical Detection and Quantitation of Cell Surface Receptors for Prostanoids -- Adhesion Signaling Through Integrins -- Electrophoretic Mobility Shift Assay (EMSA) -- Transfection Assays for Transformation of Model Colonic Cell Lines -- Assessment of Intestinal Stem Cell Survival Using the Microcolony Formation Assay -- Gene Transfer into the Colonic Mucosa.
520			_aColorectal cancer is a common and highly lethal cancer once spread whose treatment is improving as we gain a better understanding of its development. In Colorectal Cancer: Methods and Protocols, Steven M. Powell, MD, has assembled a hands-on collection of cutting-edge molecular methods for studying this solid tumor. These readily reproducible techniques range from gross microdissection of specimens to specific molecular analyses. Here both the novice and experienced researcher will find productive chromosomal analyses, including comparative genomic hybridization and FISH assays, and revealing gene expression analyses, including immunohistochemical assays, Western blotting, and microarray assays. Also presented are mutational analyses of colon tumors and blood samples to determine whether specific patients have inherited a significant predisposition to colorectal cancer. Each method is described in step-by-step detail by an established expert to ensure robust experimental results, and all are accompanied by detailed notes on pitfalls to avoid. Eminently practical, with each technique presented as if the expert in the field who had perfected it were guiding the investigator, Colorectal Cancer: Methods and Protocols provides cancer investigators with a gold standard collection of all the key genetic, cellular, and molecular biological methods for analyzing colon cancer today.
988			_aSpringer_Protocols_2001
650		7	_2embne _9182269 _aColon _xCáncer _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781617371790
776	0	8	_iPrinted edition: _z9780896037670
776	0	8	_iPrinted edition: _z9781489941985
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592590845 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eb _zSI