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008			100301s2007 xxua o |||| 0|eng d
020			_a9781597453233
024	7		_a10.1007/978-1-59745-323-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRC582 _b2007 EB
245	0	0	_aMonoclonal Antibodies : _bMethods and Protocols _cedited by Maher Albitar
250			_a1st edition 2007
264		1	_aTotowa, NJ _bHumana Press _c2007
300			_a1 recurso en línea (XII, 228 páginas) _b75 ilustraciones, 4 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v378
505	0		_aDevelopment and Characterization of Mouse Hybridomas -- Recombinant Monoclonal Antibodies -- Soluble Fc Fusion Proteins for Biomedical Research -- The Use of the Antibodies in the Diagnosis of Leukemia and Lymphoma by Flow Cytometry -- Quantification of Surface Antigens and Quantitative Flow Cytometry -- CD13 and CD10 Expression of Granulocytes as Markers for the Functioning of the Immune System -- Monitoring Cell Signaling Pathways by Quantitative Flow Cytometry -- Antibodies and Immunohistochemical Evaluation for the Diagnosis of Hematological Malignancies -- Bead-Based Multianalyte Flow Immunoassays -- Cell-Free Bead-Based Detection of Total and Phosphorylated Proteins in Plasma and Cell Lysates -- Measuring Humanized Antibodies in Plasma of Patients Treated With Antibody-Based Therapy Using Bead-Based Flow Cytometry -- Detection of Chromosome Translocations by Bead-Based Flow Cytometry -- Detection of the MDR1 P-Glycoprotein Expression and Function -- Detection of Human Antibodies Generated Against Therapeutic Antibodies Used in Tumor Therapy -- Measuring Allergen-Specific IgE.
520			_aMonoclonal Antibodies: Methods and Protocols examines a collection of state-of-the-art methods that employ monoclonal antibodies in a clinical setting with opening chapters focusing on the gold standard method for generating mouse monoclonal antibodies through hybridoma technology, future methods for engineering recombinant and humanized antibodies, methods for engineering soluble Fc fusion protein, and the use of antibodies and flow cytometry in the quantification of cell signaling proteins. Specific chapters describe how antibodies are used for the diagnosis and classification of hematologic diseases. Subsequent chapters examine the advantages and most recent advances of using bead-based immunoassays, including the ability of bead-based technology to multiplex and analyze several analytes simultaneously, and the use of beads in detecting fusion proteins resulting from chromosomal translocations. Concluding chapters provide additional examples of methodologies that employ monoclonal antibodies. Monoclonal Antibodies: Methods and Protocols provides descriptions of methods that cover a wide spectrum of applications in the field of monoclonal antibodies. This field will continue to expand and provide new and innovative techniques, not only in the laboratory, but also as a basis that complements targeted therapy.
988			_aSpringer_Protocols_2007
650		7	_2embne _9666367 _aInmunología clínica _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781617376467
776	0	8	_iPrinted edition: _z9781588295675
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-323-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eb _zSI