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008			110108s2011 xxu| o |||| 0|eng d
020			_a9781617790089
024	7		_a10.1007/978-1-61779-008-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQK725 _b2011 EB
245	0	4	_aThe Plant Cell Wall : _bMethods and Protocols _cedited by Zoë Popper
250			_a1st edition 2011
264		1	_aTotowa, NJ _bHumana Press _c2011
300			_a1 recurso en línea (XIII, 310 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v715
505	0		_aPlant Tissue Cultures -- Computerized Molecular Modelling of Carbohydrates -- Oligosaccharide Mass Profiling (OLIMP) of Cell Wall Polysaccharides by MALDI-TOF/MS -- High-Voltage Paper Electrophoresis (HVPE) of Cell-Wall Building Blocks and Their Metabolic Precursors -- Carbohydrate Gel Electrophoresis -- Capillary Electrophoresis with Detection by Laser-Induced Fluorescence -- Monoclonal antibodies, Carbohydrate-Binding Modules, and the Detection of Polysaccharides in Plant Cell Walls -- Screening and Characterization of Plant Cell Walls Using Carbohydrate Microarrays -- Electron Tomography and Immunogold Labelling as Tools to Analyze de novo Assembly of Plant Cell Walls -- Analyzing Cellulose Biosynthesis with Confocal Microscopy -- Visual Mapping of Cell Wall Biosynthesis -- Atomic Force Microscopy of Plant Cell Walls -- Using Solid-State 13C NMR Spectroscopy to Study the Molecular Organization of Primary Plant Cell Walls -- Formation of Cellulose-Based Composites with Hemicelluloses and Pectins Using Gluconacetobacter Fermentation -- Structural Proteins of the Primary Cell Wall: Extraction, Purification, and Analysis -- New Insights into the Control of Cell Growth -- Extraction and Detection of Arabinogalactan Proteins -- Characterization of the Plant Cell Wall Proteome Using High Throughput Screens -- Knocking Out the Wall: Protocols for Gene Targeting in Physcomitrella patens -- Measuring In vitro Extensibility of Growing Plant Cell Walls.
520			_aThe cell wall and its constituent polysaccharides and proteins control nearly all plant-based biological and biophysical processes. Understanding the cell wall is, therefore, not only fundamental to the plant sciences but is also pertinent to aspects of human and animal nutrition and health as well as plant-microbe and plant-animal interactions. In The Plant Cell Wall: Methods and Protocols, experts in the field describe detailed methods which are currently being applied to investigate the many aspects of the plant cell wall including its structure, biochemical composition, and metabolism.  The book delves into a range of techniques involving plant tissue culture, which can be applied to investigating cell wall structure and metabolism, methods directed towards structural analysis and occurrence of carbohydrates, the development and use of microscopy-based tools and techniques, procedures which measure the physical properties of the wall, and methods based on the application of molecular genetic approaches.  Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, The Plant Cell Wall: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this essential cellular feature.
988			_aSpringer_Protocols_2011
650		7	_2embne _9146302 _aCélulas vegetales _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781617790072
776	0	8	_iPrinted edition: _z9781617790096
776	0	8	_iPrinted edition: _z9781493961795
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-008-9 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eb _zSI