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008			140106s2014 xxua o |||| 0|eng d
020			_a9781627037822
024	7		_a10.1007/978-1-62703-782-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP551 _b2014 EB
245	0	0	_aCell-Free Protein Synthesis : _bMethods and Protocols _cedited by Kirill Alexandrov, Wayne A. Johnston
250			_a1st edition 2014
264		1	_aTotowa, NJ _bHumana Press _c2014
300			_a1 recurso en línea (XI, 313 páginas) _b66 ilustraciones, 31 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1118
505	0		_aProduction of Eukaryotic Cell-Free Lysate from Leishmania tarentolae -- Bioinformatics Analysis and Optimization of Cell-Free Protein Expression -- A Cell-Free Expression Screen to Identify Fusion Tags for Improved Protein Expression -- One-Pot, Microscale Cell-Free Enzyme Expression and Screening -- Cell-Free Translation of Biofuels Enzymes -- Cloning-Independent Expression and Screening of Enzymes using Cell-Free Protein Synthesis Systems -- High Level Cell-Free Production of Membrane Proteins with Nanodiscs -- Cell Free Protein Based Enzyme Discovery and Protein-Ligand Interaction Study -- Human Cell Extract-Derived Cell-Free Systems for Virus Synthesis -- Cell-Free Protein Synthesis in Microfluidic 96-Well Plates -- Preparation of Multiple Site-Specific Mutant Proteins for NMR Studies by PCR-Directed Cell-Free Protein Synthesis -- Site-Specific Incorporation of Unnatural Amino Acids into Proteins by Cell-Free Protein Synthesis -- In vitro Translation of Papillomavirus Authentic and Codon-Modified L1 Capsid Gene mRNAs in Mouse Keratinocyte Cell-Free Lysate -- An Optimized Yeast Cell-Free Lysate System for In vitro Translation of Human Virus mRNA -- In vitro Translation Based Protein Kinase Substrate Identification -- Preparation of Protein Arrays using Cell-Free Protein Expression -- Post-Transcriptional Control of Protein Synthesis in Drosophila S2 Cell-Free System -- Cell-Free Membrane Protein Expression -- The PURE System for Protein Production -- A Cell-Free Protein Synthesis System from Insect Cells -- A Cell-Free Expression Platform for Production of Protein Microarrays.
520			_aCell-free protein expression promises to narrow the technological gap between DNA and protein technologies and provide a platform for broad application of synthetic biology principles in the Life Sciences. It is a rapid and high throughput methodology for the conversion of DNA encoded genetic information into protein-mediated biochemical activities. Cell-Free Protein Synthesis: Methods and Protocols brings together the key opinion leaders of cell-free technology development and provides case studies and detailed protocols for the application of cell-free methodology. Chapters cover the main directions in the development of cell-free technologies including several recently developed cell-free systems, as well as a number of applications of cell-free systems ranging from discovery of biofuel enzymes to in vitro assembly of viruses.  Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Cell-Free Protein Synthesis: Methods and Protocols seeks to serve a wide variety of scientists with its well-honed methodologies.  .
988			_aSpringer_Protocols_2014
650		7	_2embne _9139861 _aProteínas _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781627037839
776	0	8	_iPrinted edition: _z9781627037815
776	0	8	_iPrinted edition: _z9781493962549
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-782-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eb _zSI