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008			161107s2017 xxua o |||| 0|eng d
020			_a9781493964420
024	7		_a10.1007/978-1-4939-6442-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP625 .N89 _b2017 EB
245	0	0	_aGenotyping : _bMethods and Protocols _cedited by Stefan J. White, Stuart Cantsilieris
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (X, 254 páginas) _b65 ilustraciones, 55 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1492
505	0		_aGenetic Fingerprinting Using Microsatellite Markers in a Multiplex PCR Reaction: A Compilation of Methodological Approaches from Primer Design to Detection Systems -- Genotyping DNA Variants with High Resolution Melting Analysis -- High-Throughput Genotyping with TaqMan Allelic Discrimination and Allele-Specific Genotyping Assays -- In Situ Single Molecule RNA Genotyping Using Padlock Probes and Rolling Circle Amplification -- The MassARRAY® System for Targeted SNP Genotyping -- Targeted Capture and High-Throughput Sequencing Using Molecular Inversion Probes (MIPs) -- Analyzing Copy Number Variation Using Pulsed-Field Gel Electrophoresis: Providing a Genetic Diagnosis for FSHD -- Analysis of Copy Number Variation Using the Paralogue Ratio Test (PRT) -- Genotyping Complex Copy Number Variation with Multiplex Ligation-Dependent Probe Amplification (MLPA) -- Analysis of Multiallelic CNVs by Emulsion Haplotype Fusion PCR -- Analysis Using Droplet Digital PCR -- Full-Length Mitochondrial-DNA Sequencing on the PacBio RSII and Full-Length MT-DNA RSII -- Targeted Locus Amplification and Next Generation Sequencing -- Efficient, Cost-Effective, High-Throughput, Multilocus Sequencing Typing (MLST) Method, NGMLST, and the Analytical Software Program MLSTEZ -- Rapid SNP Detection and Genotyping of Bacterial Pathogens by Pyrosequencing -- Methods for Genotyping-by-Sequencing.-Describing Sequence Variants Using HGVS Nomenclature.
520			_aThis volume explores a broad range of different genotyping techniques. Genotyping: Methods and Protocols consists of chapters that cover numerous topics such as: an overview of multiplexed microsatellite analysis; High Resolution Melt analysis and TaqMan-based assays; in situ analysis of variants in single RNA molecules; the MassARRAY system and Molecular Inversion Probes; Pulsed Field Gel Electrophoresis, Paralogue Ratio Test, and Multiplex Ligation-Dependent Probe Amplification; long-range PCR combined with PacBio sequencing; Targeted Locus Amplification; Multilocus Sequence Typing and rapid SNP detection with pyrosequencing; and genotyping-by-sequencing for plant analysis. Finally, the volume concludes with a summary of pertinent points to describe genetic variation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and practical, Genotyping: Methods and Protocols is a valuable resource for anyone interested in learning more about the diverse field of genotyping.
988			_aSpringer_Protocols_2017
650		7	_2embne _9144345 _aNucleótidos _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781493964406
776	0	8	_iPrinted edition: _z9781493964413
776	0	8	_iPrinted edition: _z9781493982011
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6442-0 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eb _zSI