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008			100301s2004 xxu| o |||| 0|eng d
020			_a9781592596676
024	7		_a10.1385/1592596673 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR185.8 .C95 _b2004 EB
245	0	0	_aCytokine Protocols _cedited by Marc De Ley
250			_a1st edition 2004
264		1	_aTotowa, NJ _bHumana Press _c2004
300			_a1 recurso en línea (XIII, 240 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v249
505	0		_aLarge-Scale Generation of Plasmids that Express Type I Interferon -- Identification of trans-Acting Factors by Electrophoretic Mobility Shift Assay -- Modulation of the Interferon-? Signal by Transfection of Cells with an Antisense-RNA Expressing Vector -- Competitive RT-PCR to Quantify Small Amounts of mRNA -- In SituHybridization for Cytokines in Human Tissue Biopsies -- Purification, Identification, and Synthesis of Chemokines -- Receptor Isolation and Characterization -- Crystallization of Cytokine-Receptor Complexes -- Biosensor Analysis of Receptor-Ligand Interactions -- Analysis of SH2 Ligands and Identification of Sites of Interaction -- Assays for Antiviral Activity -- Assays for Cytotoxicity -- Assays for Chemotaxis -- In Vitro and In Vivo Assays for High-Molecular-Weight Pegylated Muteins of Granulocyte Colony-Stimulating Factor -- Development of a Mammalian Tet-On Expression Cell Line -- Immunohistochemical Detection of Cytokines in Human Tissue Sections -- Detection of Cytokine- and Chemokine-Expressing Cells at the Single Cell Level -- Detection of Cytokine Signal Transduction "Cross-Talk" in Leukocyte Activation -- Assay System for the Effect of Prostaglandin E2 in the Determination of Polarized Cytokine Production.
520			_aBecause cytokines regulate many different bodily functions in living organisms, mainly at the level of cell-cell communications, there is great interest in understanding their mode of action. In Cytokine Protocols, established researchers, physicians, and clinicians present their best biochemical, cellular, and molecular techniques for unraveling and quantifying the events occurring between the initial contact of a cytokine at the membrane receptor and the eventual activation of gene transcription. Described in step-by-step detail to ensure successful experimental results, these protocols cover the large-scale generation and purification of plasmid DNA, the identification of DNA-protein interactions via the gel mobility shift assay, RNA-level phenomena, and the isolation and characterization of cytokines, cytokine-related proteins, and their interactions. The techniques used include the generation of transfectants, the immunohistochemical detection of cytokines in tissue sections, and optimized staining for cytoplasmic detection. Highlights include RT-PCR of small amounts of mRNA, in situ hybridization, biosensor analysis, measurement of biological activities and standardization, immunohistochemical and single-cell detection, and receptor isolation, characterization, and crystallization. Each protocol includes a background introduction, equipment and reagent lists, tips on troubleshooting and avoiding pitfalls, and, where needed, a discussion of the interpretation of results. Comprehensive and highly practical, Cytokine Protocols offers novice researchers a concise introduction to the principles of cytokine research, and experienced investigators all the advanced techniques and time-saving tips needed to ensure productive research.
988			_aSpringer_Protocols_2004
650		7	_2embne _9147650 _aCitoquinas
776	0	8	_iPrinted edition: _z9781617372698
776	0	8	_iPrinted edition: _z9780896039483
776	0	8	_iPrinted edition: _z9781489939395
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592596673 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eb _zSI