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008			110414s2009 xxua o |||| 0|eng d
020			_a9781603278348
024	7		_a10.1007/978-1-60327-834-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP551 _b2009 EB
245	0	0	_aPhospho-Proteomics : _bMethods and Protocols _cedited by Marjo de Graauw
250			_a1st edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (XII, 332 páginas) _b69 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v527
505	0		_aProtein Phosphorylation and 2D Gel Electrophoresis -- A High-Resolution Two Dimensional Gel- and Pro-Q DPS-Based Proteomics Workflow for Phosphoprotein Identification and Quantitative Profiling -- Differential Phosphoprotein Labelling (DIPPL) Using 32P and 33P -- Enrichment of Phosphoproteins and Peptides -- Identification of Oxidative Stress-Induced Tyrosine Phosphorylated Proteins by Immunoprecipitation and Mass Spectrometry -- Enrichment and Characterization of Phosphopeptides by Immobilized Metal Affinity Chromatography (IMAC) and Mass Spectrometry -- The Use of Titanium Dioxide Micro-Columns to Selectively Isolate Phosphopeptides from Proteolytic Digests -- Enrichment and Separation of Mono- and Multiply Phosphorylated Peptides Using Sequential Elution from IMAC Prior to Mass Spectrometric Analysis -- Use of Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) for Phosphotyrosine Protein Identification and Quantitation -- Hydrophilic Interaction Chromatography for Fractionation and Enrichment of the Phosphoproteome -- SILAC for Global Phosphoproteomic Analysis -- Quantitative Phospho-proteomics Based on Soluble Nanopolymers -- Profiling the Tyrosine Phosphorylation State Using SH2 Domains -- Phosphoprotein Analysis by Mass Spectrometry -- An Overview of the Qualitative Analysis of Phosphoproteins by Mass Spectrometry -- The Analysis of Phosphoproteomes by Selective Labelling and Advanced Mass Spectrometric Techniques -- On-Line Liquid Chromatography Electron Capture Dissociation for the Characterization of Phosphorylation Sites in Proteins -- Quantification of Protein Phosphorylation by ?LC-ICP-MS -- Reverse-Phase Diagonal Chromatography for Phosphoproteome Research -- Chemical Tagging Strategies for Mass Spectrometry-Based Phospho-proteomics -- Arrays to Study Protein-Phosphorylation -- Antibody Array Platform to Monitor Protein Tyrosine Phosphorylation in Mammalian Cells -- Protein Tyrosine Kinase Characterization Based on Fully Automated Synthesis of (Phospho) Peptide Arrays in Microplates -- Kinome Profiling Using Peptide Arrays in Eukaryotic Cells -- Bioinformatics -- ProMoST: A Tool for Calculating the pI and Molecular Mass of Phosphorylated and Modified Proteins on Two-Dimensional Gels -- Kinase-Specific Prediction of Protein Phosphorylation Sites -- Reconstructing Regulatory Kinase Pathways from Phosphopeptide Data: A Bioinformatics Approach.
520			_aProtein phosphorylation controls many basic cellular processes, such as cell growth, differentiation, migration, metabolism, and cell death, and its study can provide key insights into the signal transduction pathways that are activated in cells in response to different stimuli, such as growth factor stimulation or exposure to toxicants. In Phospho-Proteomics: Methods and Protocols, expert researchers contribute both well-established protocols and some of the newest strategies for the identification and evaluation of protein phosphorylation on Tyr, Ser, and Thr residues, including topics such as 2-dimensional gel electrophoresis and protein phosphorylation, enrichment of phospho-proteins and peptides, quantitative analysis of phosphorylation by labeling and MS analysis, and antibody and kinase arrays. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Phospho-Proteomics: Methods and Protocols is an ideal reference for both new and experienced scientists who wish to gain insight into the current developments in the field and to find inspiration to pursue its future.
988			_aSpringer_Protocols_2009
650		7	_2embne _9430647 _aProteómica _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781603278362
776	0	8	_iPrinted edition: _z9781617379178
776	0	8	_iPrinted edition: _z9781603278331
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60327-834-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
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998			_b01/2024 _dz _eb _zSI