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008			100301s2008 xxua o |||| 0|eng d
020			_a9781603270847
024	7		_a10.1007/978-1-60327-084-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH450 _b2008 EB
245	0	0	_aPost-translational Modifications of Proteins : _bTools for Functional Proteomics _cedited by Christoph Kannicht
250			_a2nd edition 2008
264		1	_aTotowa, NJ _bHumana Press _c2008
300			_a1 recurso en línea (XVIII, 390 páginas) _b128 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v446
505	0		_aDisulfide Bond Mapping by Cyanylation-induced Cleavage and Mass Spectrometry -- Detection of Post-translational Modifications by Fluorescent Staining of Two-Dimensional Gels -- Identification of Protein Phosphorylation Sites by Advanced LC-ESI-MS/MS Methods -- Analysis of Tyrosine-O-Sulfation -- ?-Amidated Peptides: Approaches for Analysis -- ?-Glutamate and ?-Hydroxyaspartate in Proteins -- Lysine Hydroxylation and Cross-linking of Collagen -- Mass Spectrometric Determination of Protein Ubiquitination -- Analysis of Sumoylation -- Detection and Analysis of Protein ISGylation -- Analysis of Methylation, Acetylation, and other Modifications in Bacterial Ribosomal Proteins -- Analysis of S-Acylation of Proteins -- Metabolic Labeling and Structural Analysis of Glycosylphosphatidylinositols from Parasitic Protozoa -- 2-Dimensional Electrophoresis: Detection of Glycosylation and Influence on Spot Pattern -- Carbohydrate Composition Analysis of Glycoproteins by HPLC Using Highly Fluorescent Anthranilic Acid (AA) Tag -- Enzymatical Hydrolysis of N-Glycans from Glycoproteins and Fluorescent Labeling by 2-Aminobenzamide (2-AB) -- Separation of N-Glycans by HPLC -- Enzymatic Sequence Analysis of N-Glycans by Exoglycosidase Cleavage and Mass Spectrometry - detection of Lewis X Structures -- Immunochemical Methods for the Rapid Screening of the O-Glycosidically Linked N-Acetylglucosamine Modification of Proteins -- Analysis of O-Glycosylation -- Characterization of Site-specific N-Glycosylation -- Monitoring Glycosylation of Therapeutic Glycoproteins for Consistency by HPLC Using Highly Fluorescent Anthranilic Acid (AA) Tag -- Comparability and Monitoring Immunogenic N-linked Oligosaccharides from Recombinant Monoclonal Antibodies from Two Different Cell Lines using HPLC with Fluorescence Detection and Mass Spectrometry -- Mass Spectrometry and HPLC with Fluorescent Detection-Based Orthogonal Approaches to Characterize N-Linked Oligosaccharides of Recombinant Monoclonal Antibodies -- Web-based Computational Tools for the Prediction and Analysis of Post-translational Modifications of Proteins.
520			_aThe majority of all proteins undergo co- and/or post-translational modifications, crucially altering physical and chemical properties, folding, conformation distribution, stability, activity, and, consequently, the function of the proteins. In Post-translational Modifications of Proteins: Tools for Functional Proteomics, Second Edition, this important area of research is brought up-to-date by the leading scientists in the field. This compilation of detailed protocols focuses on protein function, proteome research and characterization of pharmaceutical proteins, while following the successful format of the Methods in Molecular Biology™ series. Each chapter provides a brief introduction to the topic, step-by-step laboratory protocols, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting edge, Post-translational Modifications of Proteins: Tools for Functional Proteomics, Second Edition serves as practical guide for researchers working in the field of protein structure-function relationships and the rapidly growing field of proteomics, as well as scientists in the pharmaceutical industries.
988			_aSpringer_Protocols_2008
650		7	_2embne _9162770 _aExpresión génica
776	0	8	_iPrinted edition: _z9781617377396
776	0	8	_iPrinted edition: _z9781588297198
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60327-084-7 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eb _zSI