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020 _a9781592595143
024 7 _a10.1385/0896032507
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQH601
_b1994 EB
100 1 _aGraham, J. M.
_d1943-
_eautor
_4aut
_4http://id.loc.gov/vocabulary/relators/aut
_9689399
_q(John M.)
245 1 0 _aBiomembrane Protocols
_nII,
_pArchitecture and Function
_cby John M. Graham, Joan A. Higgins
250 _a1st edition 1994
264 1 _aTotowa, NJ
_bHumana Press
_c1994
300 _a1 recurso en línea (XIII, 362 páginas)
_b7 ilustraciones
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v27
505 0 _aCrystallization of Membrane Proteins for X-Ray Analysis -- Determination of Cell-Surface Polarity by Solid-Phase Lactoperoxidase Iodination -- Biochemical Methods to Determine Cell-Surface Topography -- Use of Antipeptide Antibodies for the Isolation and Study of Membrane Proteins -- The Production of Monoclonal Antibodies to Membrane Proteins -- Purification of a Membrane Protein (Ca2+/Mg2+-ATPase) and Its Reconstitution into Lipid Vesicles -- Measurement of Protein-Protein Interactions in Reconstituted Membrane Vesicles Using Fluorescence Spectroscopy -- Measurement of Lipid-Protein Interactions in Reconstituted Membrane Vesicles Using Fluorescence Spectroscopy -- Determination of the Transverse Topography of Membrane Lipids Using Enzymes and Covalent Labels as Probes -- Determination of the Transverse Topography of Membrane Phospholipids Using Phospholipid Transfer Proteins as Tools -- Prothrombinase Complex as a Tool to Assess Changes in Membrane Phospholipid Asymmetry -- Fluorescent Glycerolipid Probes -- Synthesis and Use of Spin-Labeled Lipids for Studies of the Transmembrane Movement of Phospholipids -- Measurement of Membrane Fluidity and Membrane Fusion with Fluorescent Probes -- Extraction and Assay of Cyclic Nucleotides -- Analysis of G-Proteins Regulating Signal Transduction Pathways -- Assay of Protein Kinases and Protein Phosphorylation -- Analysis of Cellular Phosphoinositides and Phosphoinositols by Extraction and Simple Analytical Procedures -- Analysis of Cellular Phosphoinositides and Phosphoinositols By High-Performance Liquid Chromatography -- Cytosolic Free Calcium Measurements in Single Cells Using Calcium-Sensitive Fluorochromes -- Membrane Permeabilization with Bacterial Toxins -- Measurement of Ion Fluxes and pH Gradients Across Cell Membranes -- Ligand Binding and Processing -- The Binding of Protein-Ligands to Cell-Surface Receptors.
520 _aThere have been many important advances in our understanding of biological membrane structure and function over the last decade. Much of this progress has been driven by the development of new techniques for studying membrane components and their interactions. Tradition­ ally, the investigation of membranes has largely occurred within the domains of biochemistry, physical chemistry, and cell biology; but many of the most significant advances have resulted from an expansion into other disciplines, such as molecular biology, immunology, and the clinical sciences. In these two volumes we have attempted to bring together some of these techniques-to combine the old and the new. Biomembrane Protocols: II. Architecture and Function is concerned exclusively with the architecture and activities of membranes, whereas the companion volume, Biomembrane Protocols: I. Isolation and Analysis, deals with their isolation and compositional analysis. The aim of each chapter is to provide detailed technical and meth­ odological information that will allow the reader to perform the tech­ nique successfully, without the need to consult other texts. Detailed reviews of membrane structure and function are not included, except where they are relevant to the choice or efficacy of a particular proce­ dure. In addition to Materials and Methods sections, each chapter has a Notes section that explains the reasons for taking certain steps and pro­ vides practical tips for applying the techniques to other systems.
988 _aSpringer_Protocols_1994
650 7 _2embne
_9142266
_aMembranas celulares
700 1 _aHiggins, Joan A.
_eautor
_4aut
_4http://id.loc.gov/vocabulary/relators/aut
_9689400
776 0 8 _iPrinted edition:
_z9781489939098
776 0 8 _iPrinted edition:
_z9781489939081
776 0 8 _iPrinted edition:
_z9780896032507
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896032507
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b01/2024
_dz
_eb
_zSI