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008			140208s2014 xxua o |||| 0|eng d
020			_a9781627039680
024	7		_a10.1007/978-1-62703-968-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP608 _b2014 EB
245	0	0	_aHoming Endonucleases : _bMethods and Protocols _cedited by David R. Edgell
250			_a1st edition 2014
264		1	_aTotowa, NJ _bHumana Press _c2014
300			_a1 recurso en línea (X, 284 páginas) _b60 ilustraciones, 39 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1123
505	0		_aHoming Endonucleases:  From Genetic Anomalies to Programmable Genomic Clippers -- Bioinformatic Identification of Homing Endonucleases and Their Target Sites -- PCR-Based Bioprospecting for Homing Endonucleases in Fungal Mitochondrial rRNA Genes -- Mapping Homing Endonuclease Cleavage Sites Using In Vitro Generated Protein -- Mapping Free-Standing Homing Endonuclease Promoters Using 5'RLM-RACE -- PCR Analysis of Chloroplast Double-Strand Break (DSB) Repair Products Induced by I-CreII in Chlamydomonas and Arabidopsis -- A Two-Plasmid Bacterial Selection System for Characterization and Engineering of Homing Endonucleases -- Rapid Screening of Endonuclease Target Site Preference Using a Modified Bacterial Two-Plasmid Selection -- A Yeast-Based Recombination Assay for Homing Endonuclease Activity.- Rapid Determination of Homing Endonuclease DNA Binding Specificity Profile -- Quantifying the Information Content of Homing Endonuclease Target Sites by Single Base Pair Profiling -- Homing Endonuclease Target Site Specificity Defined by Sequential Enrichment and Next-Generation Sequencing of Highly Complex Target Site Libraries -- Homing Endonuclease Target Determination Using SELEX Adapted for Yeast Surface Display -- Engineering and Flow-Cytometric Analysis of Chimeric LAGLIDADG Homing Endonucleases from Homologous I-OnuI-Family Enzymes -- Bioinformatics Identification of Coevolving Residues -- Identification and Analysis of Genomic Homing Endonuclease Target Sites -- Redesigning the Specificity of Protein-DNA Interactions with Rosetta.
520			_aHoming Endonucleases: Methods and Protocols aims at providing molecular biologists with a comprehensive resource to identify and characterize homing endonucleases from genomic sequence, to deduce the biological basis of binding and cleavage specificity, as well as to provide protocols to redesign endonuclease target specificity for genome-editing applications. Engineering of designer homing endonucleases has set the stage for genome editing of complex eukaryotic genomes with a broad range of potential applications including targeted gene knockouts in model organisms and gene therapy in humans, making this book a valuable resource for future research.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Homing Endonucleases: Methods and Protocols serves as a key reference for all labs studying site-specific DNA endonucleases.
988			_aSpringer_Protocols_2014
650		7	_2embne-DP _9146752 _aHidrolasas _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781627039697
776	0	8	_iPrinted edition: _z9781627039673
776	0	8	_iPrinted edition: _z9781493963195
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-968-0 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eb _zSI