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008			100301s2003 xxu| o |||| 0|eng d
020			_a9781592594085
024	7		_a10.1385/1592594085 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRC925 _b2003 EB
245	0	0	_ap53 Protocols _cedited by Sumitra Deb, Swati Palit Deb
250			_a1st edition 2003
264		1	_aTotowa, NJ _bHumana Press _c2003
300			_a1 recurso en línea (XI, 280 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v234
505	0		_aAdenovirus Expressing p53 -- Purification of Recombinant p53 from Sf9 Insect Cells -- Generation and Characterization of p53 Mutant Mice -- Identification of p53 Target Genes by Fluorescent Differential Display -- p53-Induced Gene Expression Analysis -- Real-Time Polymerase Chain Reaction Quantitation of Relative Expression of Genes Modulated by p53 Using SYBR® Green I -- Transactivation and Transrepression Studies with p53 -- Methods to Study p53-Repressed Promoters -- Interaction of p53 with Cellular Proteins -- Interaction of p53 with the Adenovirus E1B-55 kDa Protein -- In Vivo Footprinting and DNA Affinity Chromatography for Analysis of p53 DNA Binding Ability -- Signaling to p53 -- Detection of Mitochondrial Localization of p53 -- Analysis of Nuclear and Cytoplasmic Degradation of p53 in Cells after Stress -- Mutational Analysis of p53 in Human Tumors -- Mutational Analysis of p53 in Human Tumors -- Flow Cytometric Analysis of p53-Induced Apoptosis -- Flow Cytometric Analysis of MDM2-Mediated Growth Arrest.
520			_aSince the discovery of p53 as a tumor suppressor, numerous methods have evolved to reveal its unique structural features and biochemical functions. In p53 Protocols, Sumitra and Swati Palit Deb have assembled an indispensable collection of novel techniques that have proven most useful for studying the physiological properties of p53 both in vitro and in vivo. Described by leading basic and clinical researchers who have successfully used the methods, the techniques provide proven solutions to problems in studying the purification, target identification, gene expression, quantitation, interaction, signaling, transactivation, and transrepression of p53. The methods are also useful for delineating the functions of other proteins that may act as tumor or growth suppressors. Each technique includes step-by-step instructions, troubleshooting notes, a theoretical review, and discussion of associated problems that might arise during the course of investigation. Special effort has been made to discuss problems and their solutions to help ease the learning curve when standardizing a new method. Diverse and highly practical, p53 Protocols offers both beginning and experienced researchers in cancer biology a gold-standard compendium of readily reproducible techniques for studying p53 and related proteins.
988			_aSpringer_Protocols_2003
650		7	_2embne _9181875 _aAparato locomotor _xEnfermedades
776	0	8	_iPrinted edition: _z9781617373619
776	0	8	_iPrinted edition: _z9781588291066
776	0	8	_iPrinted edition: _z9781489938367
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592594085 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eb _zSI