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008			131009s2014 xxua o |||| 0|eng d
020			_a9781627036498
024	7		_a10.1007/978-1-62703-649-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQD95 _b2014 EB
245	0	0	_aFluorescence Spectroscopy and Microscopy : _bMethods and Protocols _cedited by Yves Engelborghs, Antonie J. W. G. Visser
250			_a1st edition 2014
264		1	_aTotowa, NJ _bHumana Press _c2014
300			_a1 recurso en línea (XXI, 816 páginas) _b244 ilustraciones, 171 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1076
505	0		_aPart I: Steady-State Fluorescence Spectroscopy (SSFS). How to Collect National Institute of Standards and Technology (NIST) Traceable Fluorescence Excitation and Emission Spectra -- Steady State Fluorescence Polarization/Anisotropy for the Study of Protein Interactions -- Quantitative Fluorescence Spectral Analysis of Protein Denaturation -- High-Pressure Fluorescence Applications -- Part II: Time-Resolved Fluorescence Spectroscopy (TRFS). Frequency Domain Fluorometry: Theory and Application -- Polar Plot Representation of Time-Resolved Fluorescence -- Ensemble and Single Molecule Detected Time Resolved FRET Methods in Studies of Protein Conformations and Dynamics -- MD + QM Correlations with Tryptophan Fluorescence Spectral Shifts and Lifetimes -- Analysis of Time-Dependent Red Shifts in Fluorescence Emission from Tryptophan Residues in Proteins -- Global Analysis of Time-Resolved Fluorescence Data -- Nanometrology -- Upconversion Spectrophotofluorometry -- Subpicosecond Kerr-Gate Spectrofluorometry -- Photo-Induced Electron Transfer Modeling to Simulate Flavoprotein Fluorescence Decay -- Part III: Fluorescent Probe Development (FPD). Biosynthetic Incorporation of Trp Analogs in Proteins -- Optimization of Fluorescent Proteins -- Monitoring Membrane Properties and Apoptosis Using Membrane Probes of the 3-Hydroxyflavone Family -- Part IV: Fluorescence Microscopy: Fluorescence Recovery After Photobleaching (FRAP). Rectangle FRAP for Measuring Diffusion with a Laser Scanning Microscope -- Part V: Fluorescence Microscopy: Förster Resonance Energy Transfer Imaging (FRETim). A Quantitative Protocol for Intensity-Based Live Cell FRET Imaging -- Part VI: Fluorescence Microscopy: Fluorescence Lifetime Imaging (FLIM). Widefield Fluorescence Lifetime Imaging with Multi-Anode Detectors -- Global Analysis of FRET- FLIM Data in Live Plant Cells -- Time-Resolved Fluorescence Anisotropy Imaging -- Multi-Modal Fluorescence Imaging Spectroscopy -- Part VII: Fluorescence Microscopy: Fluorescence Fluctuation Spectroscopy (FFS). Application of Fluorescence Correlation Spectroscopy (FCS) to Measure the Dynamics of Fluorescent Proteins in Living Cells -- Fluorescence Cross-Correlation Spectroscopy (FCCS) in Living Cells -- Quantifying Lipid-Protein Interaction by Fluorescence Correlation Spectroscopy (FCS) -- PET-FCS: Probing Rapid Structural Fluctuations of Proteins and Nucleic Acids by Single-Molecule Fluorescence Quenching -- Z-Scan Fluorescence Correlation Spectroscopy as a Tool for Diffusion Measurements in Planar Lipid Membranes -- Scanning Fluorescence Correlation Spectroscopy (SFCS) with a Scan Path Perpendicular to the Membrane Plane -- Implementation and Application of Pulsed Interleaved Excitation for Dual-Color FCS and RICS -- Quantitative Study of Protein-Protein Interactions in Live Cell by Dual Color Fluorescence Lifetime Cross-Correlation Spectroscopy -- Brightness Experiments -- Global Analysis of Autocorrelation Functions and Photon Counting Distributions in Fluorescence Fluctuation Spectroscopy -- Simulation of Autocorrelation Function and Photon Counting Distribution in Fluorescence Fluctuation Spectroscopy -- Part VIII: Fluorescence Microscopy: Single-Molecule Fluorescence Spectroscopy (smFS). Single-Molecule Fluorescence of Nucleic Acids -- Photoswitchable Fluorescent Proteins for Superresolution Fluorescence Microscopy Above the Diffraction Limit of Light.
520			_aReflecting the expanding field's need for reliable protocols, Fluorescence Spectroscopy and Microscopy: Methods and Protocols offers techniques from a worldwide team of experts on this versatile and vital subject.  The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, fluorescent probe development, and the various sub-categories of fluorescence microscopy, such as fluorescence recovery after photobleaching (FRAP), live cell FRET imaging (FRETim), fluorescence lifetime imaging (FLIM), fluorescence fluctuation spectroscopy (FFS), and single-molecule fluorescence spectroscopy (smFS).  Written as a part of the popular Methods in Molecular Biology series, chapters include the kind of unambiguous detail and key implementation advice that proves essential for successful results.   Comprehensive and practical, Fluorescence Spectroscopy and Microscopy: Methods and Protocols aims to guide both 'novice' and established scientists toward furthering their research with these invaluable techniques.
988			_aSpringer_Protocols_2014
650		7	_2embne _9140515 _aEspectroscopia _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781627036504
776	0	8	_iPrinted edition: _z9781627036481
776	0	8	_iPrinted edition: _z9781493963157
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-649-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eb _zSI