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020 _a9781617793820
024 7 _a10.1007/978-1-61779-382-0
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aRB147.5
_b2012 EB
245 0 0 _aMitochondrial Bioenergetics :
_bMethods and Protocols
_cedited by Carlos M. Palmeira, António J. Moreno
250 _a1st edition 2012
264 1 _aTotowa, NJ
_bHumana Press
_c2012
300 _a1 recurso en línea (XI, 333 páginas)
_b83 ilustraciones, 25 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v810
505 0 _aOverview of Mitochondrial Bioenergetics.- Evaluation of Respiration with Clark Type Electrode in Isolated Mitochondria and Permeabilized Animal Cells.-  High-Resolution Respirometry.  OXPHOS Protocols for Human Cells and Permeabilized Fibres from Small Biopsies of Human Muscle -- High Throughput Analysis of Mitochondrial Oxygen Consumption. - Modulation of Cellular Respiration by Endogenously Produced Nitric Oxide in Rat Hippocampal Slices. - Mitochondrial Membrane Potential (DY) Fluctuations Associated with the Metabolic States of Mitochondria. - Safranine as a Fluorescent Probe for the Evaluation of Mitochondrial Membrane Potential in Isolated Organelles and Permeabilized Cells -- Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured Cells.-Phenomenological Kinetic and Control Analysis of Oxidative Phosphorylation in Isolated Mitochondria.-Expression of Uncoupling Proteins in a Mammalian Cell Culture System (HEK293) and Assessment of their Protein Function.-Measurement of Proton Leak and Electron Leak in Isolated Mitochondria.-Relation between Mitochondrial Membrane Potential and ROS Formation -- Use of a Calcium-Sensitive Electrode for Studies on Mitochondrial Calcium Transport .-Imaging Mitochondrial Calcium Signalling with Fluorescent Probes and Single or Two Photon Confocal Microscopy -- Mitochondrial Permeability Transition Pore and Calcium Handling.- Imaging of Mitochondrial pH Using SNARF-1 -- Redox Equivalents and Mitochondrial Bioenergetics.-NMR Methodologies for Studying Mitochondrial Bioenergetics.-Computational Modeling of Mitochondrial Function.
520 _aIncreasing interest in mitochondrial bioenergetics is being driven by the impact of drug and environmental chemical-induced disturbances of mitochondrial function as well as hereditary deficiencies and the progressive deterioration of bioenergetic performance with age.  These initiatives have fostered the investigation of genetic and environmental influences on bioenergetics. In Mitochondrial Bioenergetics: Methods and Protocols, researchers in the field detail the practical principles and assays designed to derive quantitative assessment of each set of parameters that reflect different aspects of mitochondrial bioenergetics. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls   Authoritative and practical, Mitochondrial Bioenergetics: Methods and Protocols helps elevate the quality and rate of investigative discoveries regarding disease states associated with environmental or genetic influences on mitochondrial bioenergetics.
988 _aSpringer_Protocols_2012
650 7 _2embne
_9414723
_aPatología mitocondrial
_vManuales de laboratorio
776 0 8 _iPrinted edition:
_z9781617793813
776 0 8 _iPrinted edition:
_z9781617793837
776 0 8 _iPrinted edition:
_z9781493958375
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-382-0
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b01/2024
_dz
_eb
_zSI