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008			100301s1993 xxua o |||| 0|eng d
020			_a9781592595105
024	7		_a10.1385/0896032485 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP624 _b1993 EB
100	1		_aGriffin, Annette M. _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut _9689042
245	1	0	_aDNA Sequencing Protocols _cby Annette M. Griffin, Hugh G. Griffin
250			_a1st edition 1993
264		1	_aTotowa, NJ _bHumana Press _c1993
300			_a1 recurso en línea (XII, 392 páginas) _b32 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v23
505	0		_aDNA Sequencing -- M13 Cloning Vehicles -- Cloning into M13 -- Transfection of E. coli with M13 DNA -- M13 Phage Growth and Single-Strand DNA Preparation -- M13 Phage Growth and DNA Purification Using 96 Well Microtiter Trays -- Generation of Random Fragments by Sonication -- Generation of a Nested Set of Deletions Using Exonuclease III -- Sequential Deletions of Single-Stranded DNA -- Subcloning for DNA Sequencing -- Sequencing Using Custom Designed Oligonucleotides -- Dideoxy Sequencing Reactions Using Klenow Fragment DNA Polymerase 1 -- Dideoxy Sequencing Reactions Using T7 Polymerase -- Dideoxy Sequencing Reactions Using Sequenase Version 2.0 -- Dideoxy Sequencing Reactions Using Taq Polymerase -- Pouring Linear and Buffer-Gradient Sequencing Gels -- Electrophoresis of Sequence Reaction Samples -- Plasmid Sequencing -- Plasmid Sequencing -- Direct Sequencing of Inserts Cloned into Lambda Vectors -- Cosmid Sequencing -- Genomic Sequencing -- Sequencing of Double-Stranded PCR Products -- Sequencing Double-Stranded Linear DNA with Sequenase and [?-35S] d.ATP -- Solid Phase PCR Sequencing of Biotinylated Products -- Cycle Sequencing -- Direct Blotting Electrophoresis -- Multipllex DNA Sequencing -- DNA Sequencing by Chemiluminescent Detection -- Reverse Sequencing of M13 Cloned DNA -- Terminal Labeling of DNA for Maxam and Gilbert Sequencing -- DNA Sequencing the Chemical Method -- DNA Sequencing by Chemical Degradation Using One, Two, and Four Different Fluorophores -- Linear Amplification Sequencing with Dye Terminators -- Sequencing Reactions for the Applied Biosystems 373AAutomated DNA Sequencer -- Sequencing Reactions for ALF (EMBL) Automated DNA Sequencer -- Sequencing Using the Du Pont Genesis™ 2000 DNA Analysis System -- The Use of Robotic Workstations in DNA Sequencing.
520			_aThe purpose of DNA Sequencing Protocols is to provide detailed practical procedures for the widest range of DNA sequencing meth­ ods, and we believe that all the vanguard techniques now being applied in this fast-evolving field are comprehensively covered. Sequencing technology has advanced at a phenomenal rate since the original methods were first described in the late 1970s and there is now a huge variety of strategies and methods that can be employed to determine the sequence of any DNA of interest. More recently, a large number of new and innovative sequencing techniques have been developed, including the use of such novel polymerases as Tag poly­ merase and Sequenase, the harnessing of PCR technology for linear amplification (cycle) sequencing, and the advent of automated DNA sequencers. DNA sequencing is surely one of the most important techniques in the molecular biology laboratory. Sequence analysis is providing an increasingly useful approach to the characterization of biological systems, and major multinational projects are already underway to map and sequence the entire genome of organisms, such as Escherichia coli, Saccharomyces cerevisiae, Caenorhabditis elegans, and Homo sapiens. Most scientists recognize the importance of DNA sequence data and perceive DNA sequencing as a valuable and indispensable aspect of their work. Recent technological advances, especially in the area of automated sequencing, have removed much of the drudg­ ery that was formerly associated with the technique, whereas innova­ tive computer software has greatly simplified the analysis and manipulation of sequence data.
988			_aSpringer_Protocols_1993
650		7	_2embne _9140806 _aADN
700	1		_aGriffin, Hugh G. _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut _9689043
776	0	8	_iPrinted edition: _z9781489939968
776	0	8	_iPrinted edition: _z9781489939951
776	0	8	_iPrinted edition: _z9780896032484
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896032485 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
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998			_b01/2024 _dz _eb _zSI