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008			131107s2014 xxua o |||| 0|eng d
020			_a9781627036917
024	7		_a10.1007/978-1-62703-691-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP551 _b2014 EB
245	0	0	_aStructural Genomics : _bGeneral Applications _cedited by Yu Wai Chen
250			_a1st edition 2014
264		1	_aTotowa, NJ _bHumana Press _c2014
300			_a1 recurso en línea (XV, 358 páginas) _b80 ilustraciones, 63 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1091
505	0		_aDisMeta - a Meta Server for Construct Design and Optimization -- Stable Expression Clones and Auto-Induction for Protein Production in E. Coli -- High-Throughput Expression Screening and Purification of Recombinant Proteins in E. coli -- Medium Throughput Production of Recombinant Human Proteins: Ligation Independent Cloning -- Medium Throughput Production of Recombinant Human Proteins: Protein Production in E. coli -- Medium Throughput Production of Recombinant Human Proteins: Protein Production in Insect Cells -- OmniBac: Universal Multigene Transfer Plasmids for Baculovirus Expression Vector Systems -- Multiprotein Complex Production in Insect Cells by Using Polyproteins -- Expression Screening in Mammalian Suspension Cells -- Cell-free Expression of Protein Complexes for Structural Biology -- Cell-Free Protein Synthesis for Functional and Structural Studies -- Insoluble Protein Purification with Sarkosyl: Facts and Precautions -- Estimation of Crystallization Likelihood Through a Fluorimetric Thermal Stability Assay -- CrystalDirect™: A Novel Approach for Automated Crystal Harvesting Based on Photoablation of Thin Films -- Methods to Refine Macromolecular Structures in Cases of Severe Diffraction Anisotropy -- Applications of NMR-Based PRE and EPR-Based DEER Spectroscopy to Homodimer Chain Exchange Characterization and Structure Determination -- A Cost-Effective Protocol for the Parallel Production of Libraries of 13CH3-Specifically Labelled Mutants for NMR Studies of High Molecular Weight Proteins -- High-Throughput SAXS for the Characterization of Biomolecules in Solution: A Practical Approach -- Measuring Spatial Restraints on Native Protein Complexes using Isotope-Tagged Chemical Cross-Linking and Mass Spectrometry -- Modeling of Proteins and Their Assemblies with the Integrative Modeling Platform -- The Quality and Validation of Structures from Structural Genomics -- Navigating the Global Protein-Protein Interaction Landscape Using iRefWeb -- Mespeus,  A Database of Metal Interactions with Proteins -- High-Quality Macromolecular Graphics on Mobile Devices: A Quick Starter's Guide.
520			_aThe field of Structural Genomics has produced many technological advances that transform and accelerate structure solution and analysis. Structural Genomics: General Applications emphasizes the benefits to the wider structural research community. It also reflects the current trend in tackling the more ambitious challenges of studying macromolecular machineries and complexes. Divided into three convenient sections, topics include the cloning and production of proteins for structural studies, experimental methods, and computational methods and data analysis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Structural Genomics: General Applications aims primarily to channel spin-off technologies to the average structural biologist in a small or medium-sized laboratory.
988			_aSpringer_Protocols_2014
650		7	_2embne _9139861 _aProteínas
776	0	8	_iPrinted edition: _z9781627036924
776	0	8	_iPrinted edition: _z9781627036900
776	0	8	_iPrinted edition: _z9781493960606
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-691-7 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eb _zSI