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008			100301s2009 xxua o |||| 0|eng d
020			_a9781603275750
024	7		_a10.1007/978-1-60327-575-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH583.2 _b2009 EB
245	0	4	_aThe Nuclear Receptor Superfamily : _bMethods and Protocols _cedited by Iain J. McEwan
250			_a1st edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (240 páginas) _b43 ilustraciones, 4 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v505
505	0		_aNuclear Receptors: One Big Family -- Ligand Binding and Nuclear Receptor Turnover -- Methods for Measuring Ligand Dissociation and Nuclear Receptor Turnover in Whole Cells -- Flow Cytometry as a Tool for Measurement of Steroid Hormone Receptor Protein Expression in Leukocytes -- X-Ray Crystallography of Agonist/Antagonist-Bound Receptors -- Nuclear Localization and DNA Binding -- FRAP and FRET Methods to Study Nuclear Receptors in Living Cells -- Receptor-DNA Interactions: EMSA and Footprinting -- Chromatin Immunoprecipitation (ChIP) Methodology and Readouts -- Nuclear Receptor-Co-Regulatory Protein Interactions -- Yeast-Based Reporter Assays for the Functional Characterization of Cochaperone Interactions with Steroid Hormone Receptors -- High Throughput Analysis of Nuclear Receptor-Cofactor Interactions -- Binding Affinity and Kinetic Analysis of Nuclear Receptor/Co-Regulator Interactions Using Surface Plasmon Resonance -- Using RNA Interference to Study Protein Function -- Using Intrinsic Fluorescence Emission Spectroscopy to Study Steroid Receptor and Coactivator Protein Conformation Dynamics -- Pathophysiological Analysis of Nuclear Receptor Function -- Development of Phosphorylation Site-Specific Antibodies to Nuclear Receptors -- Tissue-Selective Knockouts of Steroid Receptors: A Novel Paradigm in the Study of Steroid Action -- Methods for Identifying and Studying Genetic Alterations in Hormone-Dependent Cancers.
520			_aActing principally to control patterns of gene expression, nuclear receptors play vital roles during embryonic development and in the regulation of metabolic and reproductive functions in adult life, which proves this superfamily of ligand-activated transcription factors to be a crucial part of biological life. In The Nuclear Receptor Superfamily: Methods and Protocols, expert researchers describe a range of molecular, structural and cell biological techniques currently used to investigate the structure-function of nuclear receptors, together with experimental approaches that may lead to new therapeutic strategies for treating nuclear receptor-associated diseases. Written in the highly successful Methods in Molecular Biology™ series format, the chapters in this volume contain brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as notes from the experts to highlight tips on troubleshooting and avoiding known pitfalls. Cutting-edge and easy to use, The Nuclear Receptor Superfamily: Methods and Protocols provides beneficial and time-saving guidance for all those undertaking research in this ever-growing field of study.
988			_aSpringer_Protocols_2009
650		7	_2embne _9139477 _aCitología _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781607610878
776	0	8	_iPrinted edition: _z9781617379130
776	0	8	_iPrinted edition: _z9781603275743
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60327-575-0 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eb _zSI