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020 _a9781592596508
024 7 _a10.1385/1592596509
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQH442
_b2004 EB
245 0 0 _aGene Delivery to Mammalian Cells
_nVolume 2,
_pViral Gene Transfer Techniques
_cedited by William C. Heiser
250 _a1st edition 2004
264 1 _aTotowa, NJ
_bHumana Press
_c2004
300 _a1 recurso en línea (XVIII, 566 páginas)
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v246
505 0 _aDelivery Using Adenoviruses -- Adenovirus-Mediated Gene Delivery -- DNA Delivery to Cells in Culture -- Adenovirus-Mediated Gene Delivery to Skeletal Muscle -- Delivery of Adenoviral DNA to Mouse Liver -- Delivery of DNA to Lung Airway Epithelium -- Delivery of DNA to Pulmonary Endothelium Using Adenoviral Vectors -- Gene Transfer to Brain and Spinal Cord Using Recombinant Adenoviral Vectors -- Adenovirus-Mediated Gene Transfer to Tumor Cells -- Adenovirus-Mediated Gene Delivery to Dendritic Cells -- Delivery Using Adeno-Associated Viruses -- Overview of Adeno-Associated Viral Vectors -- AAV Vector Delivery to Cells in Culture -- AAV-Mediated Gene Transfer to Skeletal Muscle -- AAV-Mediated Gene Transfer to the Liver -- AAV-Mediated Gene Transfer to Mouse Lungs -- Gene Delivery to the Mammalian Heart Using AAV Vectors -- Gene Delivery to the Mouse Brain with Adeno-Associated Virus -- Delivery of DNA to Tumor Cells In Vivo Using Adeno-Associated Virus -- Gene Delivery to Human and Murine Primitive Hematopoietic Stem and Progenitor Cells by AAV2 Vectors -- Delivery Using Herpes Simplex Viruses -- Delivery Using Herpes Simplex Virus -- Gene Transfer to Skeletal Muscle Using Herpes Simplex Virus-Based Vectors -- Delivery of Herpes Simplex Virus-Based Vectors to the Nervous System -- Gene Transfer to Glial Tumors Using Herpes Simplex Virus -- Delivery of Herpes Simplex Virus-Based Vectors to Stem Cells -- Delivery Using Baculoviruses -- Baculovirus-Mediated Gene Delivery into Mammalian Cells -- Delivery Using Lentiviruses -- Gene Delivery by Lentivirus Vectors -- Lentiviral Vectors for the Delivery of DNA into Mammalian Cells -- Stable Gene Delivery to CNS Cells Using Lentiviral Vectors -- Gene Delivery to Hematopoietic Stem Cells Using Lentiviral Vectors -- Delivery of Genes to the Eye Using Lentiviral Vectors -- Lentiviral Transduction of Human Dendritic Cells -- Delivery Using Retrovirus -- Gene Transfer by Retroviral Vectors -- Gene Delivery to Cells in Culture Using Retroviruses -- Retrovirus-Mediated Gene Transfer to Tumors -- Dilivery of Genes to Hematopoietic Stem Cells -- Delivery Using Alphaviruses -- Delivery and Expression of Heterologous Genes in Mammalian Cells Using Self-Replicating Alphavirus Vectors.
520 _aWhether to assess the function of new genes identified from the Human Genome Project or to apply gene therapy successfully, it is often necessary to deliver genes to specific cells. In Gene Delivery to Mammalian Cells, highly experienced researchers describe in great detail methods that have proven most useful in delivering genes to mammalian cells. Volume 2: Viral Gene Transfer Techniques details procedures for delivering genes to cells in vitro and in vivo, including the use of lentiviral vectors, adenovirus, adeno-associated viruses, alphavirus, herpes simplex virus, baculovirus, and retrovirus. Many of these techniques have only been in practice for a few years and are still being refined and updated. Some are being used not only in basic science, but also in gene therapy applications. Each protocol contains step-by-step instructions, along with background notes, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. Introductory chapters review the delivery methods presented, discussing their advantages and disadvantages, how they have been used successfully for gene delivery, and the future of their technology. An accompanying volume, Volume 1: Nonviral Gene Transfer Techniques, focuses on gene delivery by a variety of chemical and physical methods. Comprehensive and cutting-edge, Gene Delivery to Mammalian Cells, volumes 1 and 2, offers biomedical specialists in industry and academia an unrivaled collection of reliable methods for the chemical, physical, and viral delivery of genes to mammalian cells, all prepared to save laboratory time and ensure experimental success.
988 _aSpringer_Protocols_2004
650 7 _2embne
_9141520
_aIngeniería genética
776 0 8 _iPrinted edition:
_z9781617373534
776 0 8 _iPrinted edition:
_z9781588290953
776 0 8 _iPrinted edition:
_z9781489939166
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592596509
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
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998 _b01/2024
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