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020 _a9781493933020
024 7 _a10.1007/978-1-4939-3302-0
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQH585.5 .F56
_b2016 EB
245 0 0 _aImaging Flow Cytometry :
_bMethods and Protocols
_cedited by Natasha S. Barteneva, Ivan A. Vorobjev
250 _a1st edition 2016
264 1 _aNew York, NY
_bSpringer International Publishing
_c2016
300 _a1 recurso en línea (XIII, 295 páginas)
_b89 ilustraciones, 86 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1389
505 0 _aQuantitative Functional Morphology by Imaging Flow Cytometry -- Principles of Amnis Imaging Flow Cytometry -- Ultrafast Microfluidic Cellular Imaging by Optical Time-Stretch -- Applications of Imaging Flow Cytometry for Microalgae -- The Analysis of Cell Cycle, Proliferation, and Asymmetric Cell Division by Imaging Flow Cytometry -- Quantitation of Chromosome Damage by Imaging Flow Cytometry -- Fluorescent In Situ Hybridization In Suspension by Imaging Flow Cytometry -- Analysis of Nucleo-Cytoplasmic Protein Shuttling by Imaging Flow Cytometry -- Using Image-Based Flow Cytometry with a FISH-Based FlowRNA Assay to Simultaneously Detect Intracellular TNF-α Protein and mRNA in Monocytes Following LPS Stimulation -- Multiparametric Characterization of Human T-Cell Immune Synapses by InFlow Microscopy -- Studying T Cells N-Glycosylation by Imaging Flow Cytometry -- Assessment of Granulocyte Subset Activation: New Information from Image-Based Flow Cytometry -- Using Image-Based Flow Cytometry to Assess Monocyte Oxidized LDL Phagocytosis Capacity -- Screening for Drugs Against the Plasmodium falciparum Digestive Vacuole by Imaging Flow Cytometry -- Accurate Assessment of Cell Death by Imaging Flow Cytometry -- Temporal Heterogeneity in Apoptosis Determined by Imaging Flow Cytometry -- FlowCAM: Quantification and Classification of Phytoplankton by Imaging Flow Cytometry -- Detection and Characterization of Rare Circulating Endothelial Cells by Imaging Flow Cytometry -- Imaging Flow Cy tometric Analysis of Primary Bone Marrow Megakaryocytes -- Sickle Cell Imaging Flow Cytometry Assay (SIFCA).
520 _aThis detailed volume for the first time explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionized our ability to analyze cells, cellular clusters, and populations in a remarkable fashion. Beginning with an introduction to technology, the book continues with sections addressing protocols for studies on the cell nucleus, nucleic acids, and FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC protocols for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells.  Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Imaging Flow Cytometry: Methods and Protocols will be a critical source for all laboratories seeking to implement IFC in their research studies.
988 _aSpringer_Protocols_2016
650 7 _2embne
_9668358
_aCitometría de flujo
_vManuales de laboratorio
776 0 8 _iPrinted edition:
_z9781493933006
776 0 8 _iPrinted edition:
_z9781493933013
776 0 8 _iPrinted edition:
_z9781493949014
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-3302-0
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b01/2024
_dz
_eb
_zSI