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008			150430s2015 xxua o |||| 0|eng d
020			_a9781493926480
024	7		_a10.1007/978-1-4939-2648-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQK861 _b2015 EB
245	0	0	_aPlant Phosphoproteomics : _bMethods and Protocols _cedited by Waltraud X. Schulze
250			_a1st edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (X, 231 páginas) _b24 ilustraciones, 16 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1306
505	0		_aThe Plant Kinome -- Phosphatases in Plants -- Phosphoproteomics in Cereals -- Screening of Kinase Substrates Using Kinase-Knockout Mutants -- Phosphopeptide Profiling of Receptor Kinase Mutants -- Combining Metabolic 15N Labelling with Improved TandemMOAC for Enhanced Probing of the Phosphoproteome -- Kinase Activity and Specificity Assay Using Synthetic Peptides -- Absolute Quantitation of Protein Post-Translational Modification Isoform -- Phosphorylation Stoichiometry Determination in Plant Photosynthetic Membranes -- Phosphopeptide Immuno-Affinity Enrichment to Enhance Detection of Tyrosine Phosphorylation in Plants -- The Peptide Microarray ChloroPhos1.0: A Screening Tool for the Identification of Arabidopsis thaliana Chloroplast Protein Kinase Substrates -- Plant Protein Kinase Substrates Identification Using Protein Microarrays -- Targeted Analysis of Protein Phosphorylation by 2D Electrophoresis -- Computational Phosphorylation Network Reconstruction: Methods and Resources -- Computational Identification of Protein Kinases and Kinase-Specific Substrates in Plants -- Databases for Plant Phosphoproteomics -- Phosphorylation Site Prediction in Plants.
520			_aThis detailed volume addresses recent developments in phosphoproteomic techniques with a particular focus on the plant system. Over the recent decades, proteomic methods were refined to study the significance and dynamics of protein phosphorylation in various biological contexts. However, working with plant tissue imposes particular challenges to the biologist which are attributed to the rigid cell wall making protein extraction more difficult, the skewed protein abundance with Rubisco as a highly abundant protein, and a large central vacuole leading to low protein yield and increased degradative enzyme activity. The methodologies in this book seek to move beyond these issues.  Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Plant Phosphoproteomics: Methods and Protocols serves as an ideal reference for researchers investigating this vital area of plant science.
988			_aSpringer_Protocols_2015
650		7	_2embne _9139638 _aFitoquímica _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781493926473
776	0	8	_iPrinted edition: _z9781493926497
776	0	8	_iPrinted edition: _z9781493949373
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2648-0 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eb _zSI