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020			_a9781592593279
024	7		_a10.1385/1592593275 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH438.4 .B55 _b2003 EB
245	0	0	_aSingle Nucleotide Polymorphisms : _bMethods and Protocols _cedited by Pui-Yan Kwok
250			_a1st edition 2003
264		1	_aTotowa, NJ _bHumana Press _c2003
300			_a1 recurso en línea (XI, 269 páginas) _b42 ilustraciones, 18 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v212
505	0		_aSNPs: Why Do We Care? -- Denaturing High-Performance Liquid Chromatography -- SNP Detection and Allele Frequency Determination by SSCP -- Conformation-Sensitive Gel Electrophoresis -- Detection of Mutations in DNA by Solid-Phase Chemical Cleavage Method -- SNP Discovery by Direct DNA Sequencing -- Computational SNP Discovery in DNA Sequence Data -- Genotyping SNPs With Molecular Beacons -- SNP Genotyping by the ?'-Nuclease Reaction -- Genotyping SNPs by Minisequencing Primer Extension Using Oligonucleotide Microarrays -- Quantitative Analysis of SNPs in Pooled DNA Samples by Solid-Phase Minisequencing -- Homogeneous Primer Extension Assay With Fluorescence Polarization Detection -- Pyrosequencing for SNP Genotyping -- Homogeneous Allele-Specific PCR in SNP Genotyping -- Oligonucleotide Ligation Assay -- Invader Assay for SNP Genotyping -- MALDI-TOF Mass Spectrometry-Based SNP Genotyping.
520			_aSingle nucleotide polymorphisms (SNPs) have become the markers of choice in elucidating the relationship between DNA sequence variation and susceptibility to disease and have clearly become the focus of the next phase of the human genome project. In Single Nucleotide Polymorphisms: Methods and Protocols, Pui-Yan Kwok, MD, PhD, has assembled a collection of robust techniques for the difficult process of SNP discovery and genotyping. These cutting-edge protocols for mutation/SNP detection utilize denaturing high-performance liquid chromatography (dHPLC), single-strand conformation polymorphism (SSCP), conformation-sensitive gel electrophoresis (CSGE), chemical cleavage, and direct sequencing. Equally powerful and up-to-date methods are given for genotyping SNPs, including molecular beacons, the Taqman assay, single-base extension approaches, pyrosequencing, ligation, the Invader assay, and primer extension with mass spectrometry detection. Described in step-by-step detail by their inventors, each method provides extensive notes on the technical steps critical for experimental success, time-saving techniques, and tips on avoiding pitfalls. Comprehensive and authoritative, Single Nucleotide Polymorphisms: Methods and Protocols provides in a readily reproducible format all the major SNP discovery and genotyping techniques in use today, whether for using DNA diagnostics to identify a pathogen, for studying the genetic basis of human disease, or for molecular breeding programs in agriculture.
988			_aSpringer_Protocols_2003
650		7	_2embne _9162047 _aMarcadores biológicos _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781489938138
776	0	8	_iPrinted edition: _z9781489938121
776	0	8	_iPrinted edition: _z9780896039681
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592593275 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eb _zSI