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020 _a9781607613695
024 7 _a10.1007/978-1-60761-369-5
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQH588 .S83
_b2010 EB
245 0 0 _aHuman Embryonic Stem Cell Protocols
_cedited by Kursad Turksen
250 _a2nd edition 2010
264 1 _aTotowa, NJ
_bHumana Press
_c2010
300 _a1 recurso en línea (XII, 455 páginas)
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v584
505 0 _aHuman Embryo Culture and Assessment for the Derivation of Embryonic Stem Cells (ESC) -- Derivation of Human Embryonic Stem Cell Lines from Vitrified Human Embryos -- Growth of Human Embryonic Stem Cells Using Derivates of Human Fibroblasts -- In Vitro Neural Differentiation of Human Embryonic Stem Cells Using a Low-Density Mouse Embryonic Fibroblast Feeder Protocol -- Optimization of Physiological Xenofree Molecularly Defined Media and Matrices to Maintain Human Embryonic Stem Cell Pluripotency -- Serum-Free and Feeder-Free Culture Expansion of Human Embryonic Stem Cells -- Single Cell Enzymatic Dissociation of Human Embryonic Stem Cells: A Straightforward, Robust, and Standardized Culture Method -- Monitoring Stemness in Long-Term hESC Cultures by Real-Time PCR -- Culture and Preparation of Human Embryonic Stem Cells for Proteomics-Based Applications -- A Two- and Three-Dimensional Approach for Visualizing Human Embryonic Stem Cell Differentiation -- Immunoflourescence and mRNA Analysis of Human Embryonic Stem Cells (hESCs) Grown Under Feeder-Free Conditions -- Study of Gap Junctions in Human Embryonic Stem Cells -- hESC Engineering by Integrase-Mediated Chromosomal Targeting -- Human Embryonic Stem Cell Differentiation on Periodontal Ligament Fibroblasts -- Generation of Neural Crest Cells and Peripheral Sensory Neurons from Human Embryonic Stem Cells -- Embryonic Stem Cells as a Model for Studying Melanocyte Development -- In Vitro Derivation of Chondrogenic Cells from Human Embryonic Stem Cells -- Vascular Differentiation of Human Embryonic Stem Cells in Bioactive Hydrogel-Based Scaffolds -- Differentiation of Neural Precursors and Dopaminergic Neurons from Human Embryonic Stem Cells -- Transplantation of Human Embryonic Stem Cells and Derivatives to the Chick Embryo -- Genetic Manipulation of Human Embryonic Stem Cells -- Genetic Manipulation of Human Embryonic Stem Cells in Serum and Feeder-Free Media -- Human-Induced Pluripotent Stem Cells: Derivation, Propagation, and Freezing in Serum- and Feeder Layer-Free Culture Conditions.
520 _aDespite political and ethical controversies surrounding the study of human embryonic stem (hES) cells, new freedoms in regard to using them for research has allowed interest to remain high in understanding the regulatory mechanisms of stem cell self-renewal, their differentiation along various lineages, and their potential use in regenerative medicine. In Human Embryonic Stem Cell Protocols, Second Edition, internationally respected researchers expand upon the popular first edition and describe in detail their most useful techniques for the molecular and cellular manipulation of these intriguing cells. This diverse collection of readily reproducible methods has been optimized for the derivation, characterization, and differentiation of hES cells, with special attention given to regenerative medicine applications. As a volume of the Methods in Molecular Biology™ series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Human Embryonic Stem Cell Protocols, Second Edition offers both novice and expert researchers powerful tools essential to understanding the maintenance and differentiation of human embryonic stem cells, as well as their applications in regenerative medicine today.
988 _aSpringer_Protocols_2010
650 7 _2embne
_9160528
_aCélulas madre
776 0 8 _iPrinted edition:
_z9781607613701
776 0 8 _iPrinted edition:
_z9781607613688
776 0 8 _iPrinted edition:
_z9781493961078
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60761-369-5
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b01/2024
_dz
_eb
_zSI