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020 _a9781493968282
024 7 _a10.1007/978-1-4939-6828-2
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aRG135
_b2017 EB
245 0 0 _aCryopreservation of Mammalian Gametes and Embryos :
_bMethods and Protocols
_cedited by Zsolt Peter Nagy, Alex C. Varghese, Ashok Agarwal
250 _a1st edition 2017
264 1 _aNew York, NY
_bSpringer International Publishing
_c2017
300 _a1 recurso en línea (XII, 372 páginas)
_b145 ilustraciones, 119 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1568
505 0 _aHistorical Background on Gamete and Embryo Cryopreservation -- Utility of Animal Models for Human Ovarian Tissue Cryopreservation -- Current Challenges in Immature Oocyte Cryopreservation -- Role of Antioxidants and Antifreeze Proteins in Cryopreservation/Vitrification -- Slow Freezing of Human Sperm -- Technology of Aseptic Cryoprotectant-free Vitrification of Human ICSI Spermatozoa -- Human Epididymal and Testicular Sperm Cryopreservation -- Human Oocytes Slow-rate Freezing: Methodology -- Slow Freezing and Thawing of Human Cleavage Stage Embryos -- Human Oocyte Vitrification -- Human Embryo Vitrification -- Human Ovarian Tissue Slow Freezing -- Human Ovarian Tissue Vitrification -- Establishing an Oocyte Cryo Bank Network -- Development of a Nationwide Network for Ovarian Tissue Crypreservation -- Directional Freezing of Ovarian Tissue and Freeze-drying of Stem Cells for Fertility Preservation -- Sterile Plate-Based Vitrification of Adherent Human Pluripotent Stem Cells and Their Derivatives Using the TWIST Method -- Vitrification: A Reliable Method for Cryopreservation of Animal Embryos -- Cryopreservation Effect on Genetic Function: Neonatal Outcomes -- Gavi-Automated Vitrification Instrument -- A. Cryotec® Vitrification Thawing -- B. Solid Surface Vitrification -- C. Automated Vitrification of Mammalian Embryos on a Digital Microfluidic Device -- D. Irvine Scientific® Vitrification System -- E. Rapid-i: Closed Vitrification Device by Vitrolife -- F. Quinn's Advantage Embryo Freeze Kit -- G. Vitrification of Blastocysts Using VitriBlast™ and ThermoBlast™ - Nidacon.
520 _aThis volume provides a comprehensive and technical presentation of numerous aspects of reproductive cell tissue cryopreservation, and presents readers with current procedures and detailed discussions of novel techniques and the latest innovations. The chapters in this book are divided into five parts and cover subjects such as: immature oocyte cryopreservation, human sperm vitrification and slow-freezing, directional freezing of ovarian tissue, automated vitrification systems, and detailed protocols on popular and commercially available cryopreservation/vitrification systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Cryopreservation of Mammalian Gametes and Embryos: Methods and Protocols includes chapters written by leading experts in the field and is a valuable resource for anyone interested in the field of cryopreservation.
988 _aSpringer_Protocols_2017
650 7 _2embne
_9160012
_aFecundación in vitro
_vManuales de laboratorio
776 0 8 _iPrinted edition:
_z9781493968268
776 0 8 _iPrinted edition:
_z9781493968275
776 0 8 _iPrinted edition:
_z9781493983100
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6828-2
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b01/2024
_dz
_eb
_zSI