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008			100301s1997 xxu| o |||| 0|eng d
020			_a9781592595501
024	7		_a10.1385/0896033538 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP625 .N89 _b1997 EB
245	0	0	_aProtein Sequencing Protocols _cedited by Bryan John Smith
250			_a1st edition 1997
264		1	_aTotowa, NJ _bHumana Press _c1997
300			_a1 recurso en línea (XIV, 375 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v64
505	0		_aStrategies for Handling Polypeptides on a Microscale -- SDS Polyacrylamide Gel Electrophoresis for N-Terminal Protein Sequencing -- Two-Dimensional Polyacrylamide Gel Electrophoresis for the Separation of Proteins for Chemical Characterization -- Electroblotting of Proteins from Polyacrylamide Gels for Chemical Characterization -- Enzymatic Cleavage of Polypeptides -- Chemical Cleavage of Polypeptides -- Preparation of Glycopeptides -- Selective Isolation of the Carboxy Terminal Peptide from a Protein -- Selective Isolation of the Amino-Terminal Peptide from ?-Amino Blocked Protein -- Analytical and Micropreparative Capillary Electrophoresis of Peptides -- Reverse-Phase HPLC Purification of Peptides from Natural Sources for Structural Analysis -- High-Performance Liquid Chromatography On-Line Derivative Spectroscopy for the Characterization of Peptides with Aromatic Amino Acid Residues -- Hydrolysis of Samples for Amino Acid Analysis -- Amino Acid Analysis -- Postcolumn Amino Acid Analysis -- Fast-Atom Bombardment and Electrospray Mass Spectrometry of Peptides, Proteins, and Glycoproteins -- Peptide Mass Fingerprinting Using MALDI-TOF Mass Spectrometry -- N-Terminal Protein Ladder Sequencing -- The Dansyl-Edman Method for Manual Peptide Sequencing -- The Dansyl Method for Identifying N-Terminal Amino Acids -- Identification of PTH-Amino Acids by High-Performance Liquid Chromatography -- Protein Sequencer Maintenance and Troubleshooting -- Identifying the Carboxy Terminal Amino Acid of a Protein -- Enzymatic and Chemical Methods for Manual C-Terminal Peptide Sequencing -- Automated Methods for C-Terminal Protein Sequencing -- Chemical Modification of Proteins for Sequence Analysis -- On-Membrane Deblocking of Proteins -- Analysis of Sites of Protein Phosphorylation -- Identification of Sites of Glycosylation -- Quantification and Location of Disulfide Bonds in Proteins.
520			_aBryan Smith draws together in Protein Sequencing Protocols a timely collection of current and state-of-the-art techniques for protein and peptide preparation and sequencing-all described by hands-on masters of the procedures. These well-tested methods convey valuable lessons and insights into using automated sequencers and/or mass spectrometers, preparing proteins and peptides on a microgram and submicrogram scale, mapping peptides, analyzing amino acid by pre- and post-column derivatization methods, and sequencing N- and C-terminal peptides by classical and newly developed techniques. They also deal with such troublesome problems as identifying sites of modification and handling very small samples. Protein Sequencing Protocols offers self-contained, step-by-step recipes that ensure easily reproducible results for those already doing sequencing, and for those who are still learning the methods, as well as for those already in one area but wishing to learn associated techniques. Its multiplicity of proven protocols will guide the challenged experimenter to successful results.
988			_aSpringer_Protocols_1997
650		7	_2embne _9144345 _aNucleótidos
776	0	8	_iPrinted edition: _z9781489940544
776	0	8	_iPrinted edition: _z9780896033535
776	0	8	_iPrinted edition: _z9781489940537
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896033538 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eb _zSI