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008			160322s2016 xxua o |||| 0|eng d
020			_a9781493934140
024	7		_a10.1007/978-1-4939-3414-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR399 _b2016 EB
245	0	0	_aAnimal Coronaviruses _cedited by Leyi Wang
250			_a1st edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (X, 218 páginas) _b25 ilustraciones, 15 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aSpringer Protocols Handbooks _x1949-2456
505	0		_aAnimal Coronaviruses: A Brief Introduction -- Immunohistochemical Staining for Detection of Porcine Epidemic Diarrhea Virus in Tissues -- Virus Neutralization Assay for Turkey Coronavirus Infection -- Recombinant Turkey Coronavirus Nucleocapsid Protein Expressed in Escherichia coli -- Antibody-Capture Enzyme-Linked Immunosorbent Assay for Detection of Antibody to Turkey Coronavirus Using Infectious Bronchitis Virus or Recombinant Nucleocapsid Protein as Coating Antigen -- Reverse Genetics of Avian Coronavirus Infectious Bronchitis Virus -- Vaccinia Virus-Based Reverse Genetics for Feline Coronaviruses -- Real-Time RT-PCR Detection of Equine Coronavirus -- Detection of Bovine Coronavirus by Conventional Reverse Transcription Polymerase Chain Reaction -- Real-Time Reverse Transcription Polymerase Chain Reaction for Rapid Detection of Transmissible Gastroenteritis Virus -- An RT-PCR Assay for Detection of Infectious Bronchitis Coronavirus Serotypes -- A Multiplex Polymerase Chain Reaction for Differential Detection of Turkey Coronavirus from Chicken Infectious Bronchitis Virus and Bovine Coronavirus -- Real-Time Reverse Transcription-polymerase Chain Reaction for Detection and Quantitation of Turkey Coronavirus RNA in Feces and Intestine Tissues -- PCR Amplification and Sequencing Analysis of Full-Length Turkey Coronavirus Spike Gene -- Feline Coronavirus RT-PCR Assays for Feline Infectious Peritonitis Diagnosis -- Genotype-Specific Detection of Ferret Coronavirus by Conventional and Real-Time Reverse Transcription Polymerase Chain Reaction -- Molecular Characterization of Canine Coronavirus -- Whole Genome Sequencing of Porcine Epidemic Diarrhea Virus by Illumina MiSeq Platform -- Next Generation Sequencing for Porcine Coronaviruses.
520			_aThis detailed volume provides diagnosticians and researchers with practical methodologies and approaches to tackle animal coronaviruses. It explores conventional immunohistochemistry, virus neutralization, enzyme-linked immunosorbent assays, expression and purification of recombinant viral proteins, and various molecular assays, including conventional and real-time reverse transcription-PCR, reverse genetics methodology, and next generation sequencing and sequence analyses. As part of the Springer Protocols Handbooks series, chapters contain readily reproducible laboratory protocols as well as expert tips on troubleshooting and avoiding known pitfalls.  Practical and authoritative, Animal Coronaviruses serves as an ideal reference for researchers examining a wide variety of coronavirus species in the Coronaviridae.
988			_aSpringer_Protocols_2016
650		7	_2embne _9688509 _aCoronavirus
776	0	8	_iPrinted edition: _z9781493934126
776	0	8	_iPrinted edition: _z9781493934133
776	0	8	_iPrinted edition: _z9781493980444
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-3414-0 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eb _zSI