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008			100301s1994 xxu| o |||| 0|eng d
020			_a9781592595167
024	7		_a10.1385/0896032892 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH600 _b1994 EB
245	0	0	_aChromosome Analysis Protocols _cedited by John R. Gosden
250			_a1st edition 1994
264		1	_aTotowa, NJ _bHumana Press _c1994
300			_a1 recurso en línea (XI, 509 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v29
505	0		_aMitotic Metaphase Chromosome Preparation from Peripheral Blood for High Resolution -- Chromosome Preparation from Hematological Malignancies -- Meiotic Chromosome Preparation -- Preparation of Chromosomes for Scanning Electron Microscopy -- Immortalized Cell Lines -- Chromosome Banding and Identification Absorption Staining -- Chromosome Banding and Identification -- Chromosome Banding -- Inhibition of Chromosome Condensation -- Analysis of Chromosomes with Restriction Endonucleases and DNase Hypersensitivity -- Automatic Karyotype Analysis -- Bivariate Chromosome Analysis Using a Commercial Flow Cytometer -- Chromosome Sorting by Flow Cytometry -- Electrophoretic Karyotype Analysis Pulsed Field Gel Electrophoresis -- Immunofluorescence Techniques Applied to Mitotic Chromosome Preparations -- Immunocytochemical Techniques Applied to Meiotic Chromosomes -- Immortalized Cell Lines -- Oligonucleotide Primed in Situ DNA Synthesis (PRINS) -- Electron Microscopic Localization of in Situ Hybrids -- Protocols for Chromosome-Mediated Gene Transfer -- Construction of Chromosome-Specific Libraries of Yeast Artificial Chromosome Recombinants from Somatic Hybrid Cell Lines -- Yeast Artificial Chromosome Recombinants in a Global Strategy for Chromosome Mapping -- Chromosome Dissection and Cloning -- Molecular Analysis of Chromosome Aberrations in Hematological Malignancies -- Molecular Analysis of Chromosome Aberrations -- Alu- and LI-Primed PCR-Generated Probes for Nonisotopie in Situ Hybridization -- Chromosome Substructure Investigation.
520			_aChromosomes, as the genetic vehicles, provide the basic material for a large proportion of genetic investigations, from the construction of gene maps and models of chromosome organization, to the inves­ tigation of gene function and dysfunction. The study of chromosomes has developed in parallel with other aspects of molecular genetics, beginning with the first preparations of chromosomes from animal cells, through the development of banding techniques, which permitted the unequivocal identification of each chromosome in a karyotype, to the present analytical methods of molecular cytogenetics. Although some of these techniques have been in use for many years, and can be learned relatively easily, most published scientific reports-as a result of pressure on space from editors, and the response to that pressure by authors-contain little in the way of technical detail, and thus are rarely adequate for a researcher hoping to find all the necessary information to embark on a method from scratch. A new user needs not only a detailed description of the methods, but also some help with problem solving and sorting out the difficulties en­ countered in handling any biological system. This was the require­ ment to which the series Methods in Molecular Biology is addressed, and Chromosome Analysis Protocols forms a part of this series.
988			_aSpringer_Protocols_1994
650		7	_2embne _9139992 _aCromosomas
776	0	8	_iPrinted edition: _z9781617370052
776	0	8	_iPrinted edition: _z9780896032439
776	0	8	_iPrinted edition: _z9781489939982
776	0	8	_iPrinted edition: _z9780896032897
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896032892 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eb _zSI