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020 _a9781617795275
024 7 _a10.1007/978-1-61779-527-5
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQR185.8 .L48
_b2012 EB
245 0 _aLeucocytes :
_bMethods and Protocols
_cedited by Robert B. Ashman
250 _a1st edition 2012
264 1 _aTotowa, NJ
_bHumana Press
_c2012
300 _a1 recurso en línea (XI, 296 páginas)
_b62 ilustraciones, 24 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v844
505 0 _aENU Based Phenotype Driven Screening -- Detection and Quantification of Cytokines and Other Biomarkers -- FLOW CYTOMETRY ANALYSIS OF CELL CYCLING AND PROLIFERATION IN MOUSE HEMATOPOIETIC STEM AND PROGENITOR CELLS -- Flow Cytometry Measurement of Bone Marrow Perfusion in the Mouse and Sorting of Progenitors and Stems Cells According to Position Relative to Blood Flow in Vivo -- Analyzing Cell Death Events in Cultured Leukocytes -- Towards a four-dimensional view of neutrophils -- Isolation of Human and Mouse Neutrophils Ex vivo and In vitro -- MEASUREMENT OF OXIDATIVE BURST IN NEUTROPHILS -- Measurement of Neutrophil Elastase, Proteinase 3 and Cathepsin G Activities using Intramolecularly Quenched Fluorogenic Substrates -- The Macrophage -- Generation and Characterization of MacGreen Mice, the Cfs1r-EGFP Transgenic Mice -- Generation of Mouse Bone Marrow-derived Macrophages -- Isolation and Differentiation of Monocytes-macrophages from Human Blood -- In Vitro Measurement of Phagocytosis and Killing of Cryptococcus neoformans by Macrophages -- Measuring the Inflammasome -- Arginine and Macrophage Activation -- Immunodetection of Granzyme B Tissue Distribution and Cellular Localisation -- Detection of Human and Mouse Granzyme B Activity in Cell Extracts -- T cell Transfer Model of Colitis: A Great Tool to Assess the Contribution of T cells in Chronic Intestinal Inflammation -- Measurement of Nitrite in Urine by Gas Chromatography-Mass Spectrometry.
520 _aThe innate immune response is a crucial component of early resistance to infection, and it is now revealing increasing levels of complexity. The ability to modify the genome in vivo, has facilitated understanding of complex interactions between leucocytes and other components of the immune system, and phenotype-driven strategies using chemical mutagenesis have placed another powerful weapon in the armamentarium. In, Leucocytes: Methods and Protocols provides detailed protocols and practical advice on a variety of modern approaches to the study of leucocytes and their products.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Leucocytes:Methods and Protocols  seeks to aid scientists in further study into the immune system and leucocytes.
988 _aSpringer_Protocols_2012
650 7 _2embne
_9688507
_aLeucocitos
_vManuales de laboratorio
776 0 8 _iPrinted edition:
_z9781617795268
776 0 8 _iPrinted edition:
_z9781617795282
776 0 8 _iPrinted edition:
_z9781493962310
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-527-5
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b12/2023
_dz
_eb
_zSI